UniProt Accession

 MTPN_HUMAN; P58546 

Genbank Protein ID

 AC015987; BC028093 

Genbank Nucleotide ID

 AAH28093.1 

Protein Name

 Myotrophin 

Protein Synonyms/Alias

 Protein V-1 

Gene Name

 MTPN 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MCDKEFMWALK	acetylation	[1]
4	****MCDKEFMWALK	ubiquitination	[2, 3]
11	KEFMWALKNGDLDEV	acetylation	[1]
11	KEFMWALKNGDLDEV	ubiquitination	[4]
24	EVKDYVAKGEDVNRT	acetylation	[1, 5]
24	EVKDYVAKGEDVNRT	ubiquitination	[6]
66	ADINAPDKHHITPLL	acetylation	[5]
66	ADINAPDKHHITPLL	ubiquitination	[3]
97	KGADKTVKGPDGLTA	acetylation	[1]
97	KGADKTVKGPDGLTA	ubiquitination	[6]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Promotes dimerization of NF-kappa-B subunits and regulates NF-kappa-B transcription factor activity (By similarity). Plays a role in the regulation of the growth of actin filaments. Inhibits the activity of the F-actin-capping protein complex formed by the CAPZA1 and CAPZB heterodimer. Promotes growth of cardiomyocytes, but not cardiomyocyte proliferation. Promotes cardiac muscle hypertrophy. 

Sequence Annotation

 REPEAT 2 30 ANK 1.
 REPEAT 34 66 ANK 2.
 REPEAT 67 99 ANK 3.
 MOD_RES 2 2 N-acetylcysteine.
 MOD_RES 4 4 N6-acetyllysine.
 MOD_RES 11 11 N6-acetyllysine.
 MOD_RES 24 24 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ANK repeat; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 118 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IEA:Compara.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0008290; C:F-actin capping protein complex; IDA:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
 GO:0006584; P:catecholamine metabolic process; IEA:Compara.
 GO:0016049; P:cell growth; IDA:UniProtKB.
 GO:0021707; P:cerebellar granule cell differentiation; IEA:Compara.
 GO:0030182; P:neuron differentiation; NAS:UniProtKB.
 GO:0010613; P:positive regulation of cardiac muscle hypertrophy; IMP:UniProtKB.
 GO:0030307; P:positive regulation of cell growth; ISS:UniProtKB.
 GO:0010557; P:positive regulation of macromolecule biosynthetic process; ISS:UniProtKB.
 GO:0051092; P:positive regulation of NF-kappaB transcription factor activity; ISS:UniProtKB.
 GO:0051247; P:positive regulation of protein metabolic process; ISS:UniProtKB.
 GO:2000812; P:regulation of barbed-end actin filament capping; IDA:UniProtKB.
 GO:0016202; P:regulation of striated muscle tissue development; NAS:UniProtKB.
 GO:0006417; P:regulation of translation; NAS:UniProtKB.
 GO:0043403; P:skeletal muscle tissue regeneration; IEA:Compara.
 GO:0051146; P:striated muscle cell differentiation; IEA:Compara. 

Interpro

 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom. 

Pfam

 PF12796; Ank_2 

SMART

 SM00248; ANK 

PROSITE

 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 

PRINTS

 PR01415; ANKYRIN.