UniProt Accession

 REP_TGMVY; P03567 

Genbank Protein ID

 K02029 

Genbank Nucleotide ID

  

Protein Name

 Replication-associated protein 

Protein Synonyms/Alias

 Rep; Protein AC1; Protein AL1 

Gene Name

 AC1, AL1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Tomato golden mosaic virus (strain Yellow vein) (TGMV) 

NCBI Taxa ID

 223341 

Lysine Modification

Position	Peptide	Type	References
68	VLIQFEGKYCCQNQR	sumoylation	[1]
102	AKSSSDVKTYIDKDG	sumoylation	[1]

Reference

 [1] Interaction between geminivirus replication protein and the SUMO-conjugating enzyme is required for viral infection.
 Sánchez-Durán MA, Dallas MB, Ascencio-Ibañez JT, Reyes MI, Arroyo-Mateos M, Ruiz-Albert J, Hanley-Bowdoin L, Bejarano ER.
 J Virol. 2011 Oct;85(19):9789-800. [PMID: 21775461] 

Functional Description

 Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep- catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities (By similarity). 

Sequence Annotation

 NP_BIND 223 230 ATP (Potential).
 REGION 144 154 Binding to RBR1.
 REGION 157 177 Oligomerization.
 MOTIF 16 20 RCR-1.
 MOTIF 58 63 RCR-2.
 MOTIF 104 107 RCR-3.
 ACT_SITE 104 104 For DNA cleavage activity (By
 METAL 50 50 Divalent metal cation (Potential).
 METAL 58 58 Divalent metal cation (Potential).
 METAL 60 60 Divalent metal cation (Potential).
 METAL 108 108 Divalent metal cation (Potential).  

Keyword

 ATP-binding; Complete proteome; Covalent protein-DNA linkage; DNA replication; DNA-binding; Endonuclease; Helicase; Host nucleus; Host-virus interaction; Hydrolase; Metal-binding; Multifunctional enzyme; Nuclease; Nucleotide-binding; Nucleotidyltransferase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 352 AA 

Protein Sequence

Gene Ontology

 GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
 GO:0019028; C:viral capsid; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0016888; F:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; IEA:InterPro.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
 GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR001301; Gemini_AL1_CLV.
 IPR001191; Gemini_AL1_REP.
 IPR022690; Gemini_AL1_REP_cat-dom.
 IPR022692; Gemini_AL1_REP_central. 

Pfam

 PF00799; Gemini_AL1
 PF08283; Gemini_AL1_M 

SMART

  

PROSITE

  

PRINTS

 PR00227; GEMCOATAL1.
 PR00228; GEMCOATCLVL1.