CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-040844
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 GTPase-activating protein and VPS9 domain-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 GAPVD1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
987VAEDILDKYRNAIKRacetylation[1]
1132FDNRTCRKLLASIAEubiquitination[2]
1195VRLLLESKEKKIREFubiquitination[2]
1261SVMNRIFKLAFYPNQubiquitination[2, 3, 4, 5]
1287EHIQRLSKVVTANHRubiquitination[2, 5]
1322IRTISAYKTPRDKVQubiquitination[2]
1327AYKTPRDKVQCILRMubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1412 AA 
Protein Sequence
MVKLDIHTLA HHLKQERLYV NSEKQLIQRL NADVLKTAEK LYRTAWIAKQ QRINLDRLII 60
TSAEASPAEC CQHAKILEDT QFVDGYKQLG FQETAYGEFL SRLRENPRLI ASSLVAGEKL 120
NQENTQSVIY TVFTSLYGNC IMQEDESYLL QVLRYLIEFE LKESDNPRRL LRRGTCAFSI 180
LFKLFSEGLF SAKLFLTATL HEPIMQLLVE DEDHLETDPN KLIERFSPSQ QEKLFGEKGS 240
DRFRQKVQEM VESNEAKLVA LVNKFIGYLK QNTYCFPHSL RWIVSQMYKT LSCVDRLEVG 300
EVRAMCTDLL LACFICPAVV NPEQYGIISD APINEVARFN LMQVGRLLQQ LAMTGSEEGD 360
PRTKSSLGKF DKSCVAAFLD VVIGGRAVET PPLSSVNLLE GLSRTVVYIT YSQLITLVNF 420
MKSVMSGDQL REDRMALDNL LANLPPAKPG KSSSLEMTPY NTPQLSPATT PANKKNRLPI 480
ATRSRSRTNM LMDLHMDHEG SSQETIQEVQ PEEVLVISLG TGPQLTPGMM SENEVLNMQL 540
SDGGQGDVPV DENKLHGPSN RSNSVSSLDL EGESVSELGA GPSGSNGVEA LQLLEHEQAT 600
TQDNLDDKLR KFEIRDMMGL TDDRDISETV SETWSTDVLG SDFDPNIDED RLQEIAGAAA 660
ENMLGSLLCL PGSGSVLLDP CTGSTISETT SEAWSVEVLP SDSEAPDLKQ EERLQELESC 720
SGLGSTSDDT DVREVSSRPS TPGLSVVSGI SATSEDIPNK IEDLRSECSS DFGGKDSVTS 780
PDMDEITHGA HQLTSPPSQS ESLLAMFDPL SSHEGASAVV RPKVHYARPS HPPPDPPILE 840
GAVGGNEARL PNFGSHVLTP AEMEAFKQRH SYPERLVRSR SSDIVSSVRR PMSDPSWNRR 900
PGNEERELPP AAAIGATSLV AAPHSSSSSP SKDSSRGETE ERKDSDDEKS DRNRPWWRKR 960
FVSAMPKDDP SPRLSAQAQV AEDILDKYRN AIKRTSPSDG AMANYESTEV MGDGESAHDS 1020
PRDEALQNIS ADDLPDSASQ AAHPQDSAFS YRDAKKKLRL ALCSADSVAF PVLTHSTRNG 1080
LPDHTDPEDN EIVCFLKVQI AEAINLQDKN LMAQLQETMR CVCRFDNRTC RKLLASIAED 1140
YRKRAPYIAY LTRCRQGLQT TQAHLERLLQ RVLRDKEVAN RYFTTVCVRL LLESKEKKIR 1200
EFIQDFQKLT AADDKTAQVE DFLQFLYGAM AQDVIWQNAS EEQLQDAQLA IERSVMNRIF 1260
KLAFYPNQDG DILRDQVLHE HIQRLSKVVT ANHRALQIPE VYLREAPWPS AQSEIRTISA 1320
YKTPRDKVQC ILRMCSTIMN LLSLANEDSV PGADDFVPVL VFVLIKANPP CLLSTVQYIS 1380
SFYASCLSGE ESYWWMQFTA AVEFIKTIDD RK 1412 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005096; F:GTPase activator activity; IEA:InterPro.
 GO:0043547; P:positive regulation of GTPase activity; IEA:GOC.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; IEA:InterPro.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR001936; RasGAP.
 IPR008936; Rho_GTPase_activation_prot.
 IPR003123; VPS9.
 IPR013995; VPS9_subgr. 
Pfam
 PF00616; RasGAP
 PF02204; VPS9 
SMART
 SM00167; VPS9 
PROSITE
 PS50018; RAS_GTPASE_ACTIV_2
 PS51205; VPS9 
PRINTS