UniProt Accession

 RRF_ECOLI; P0A805; P16174 

Genbank Protein ID

 J05113; D13334; U70214; U00096; AP009048; M69029 

Genbank Nucleotide ID

 AAA24607.1; BAA02599.1; AAB08601.1; AAC73283.1; BAB96748.1 

Protein Name

 Ribosome-recycling factor 

Protein Synonyms/Alias

 RRF; Ribosome-releasing factor 

Gene Name

 frr 

Gene Synonyms/Alias

 rrf; b0172; JW0167 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
7	*MISDIRKDAEVRMD	acetylation	[1]
15	DAEVRMDKCVEAFKT	acetylation	[1, 2]
21	DKCVEAFKTQISKIR	acetylation	[1]
26	AFKTQISKIRTGRAS	acetylation	[1]
66	VEDSRTLKINVFDRS	acetylation	[1]
80	SMSPAVEKAIMASDL	acetylation	[1]
111	PLTEERRKDLTKIVR	acetylation	[1]
115	ERRKDLTKIVRGEAE	acetylation	[1]
138	VRRDANDKVKALLKD	acetylation	[1]
140	RDANDKVKALLKDKE	acetylation	[1]
146	VKALLKDKEISEDDD	acetylation	[1]
162	RSQDDVQKLTDAAIK	acetylation	[1, 2, 3]
169	KLTDAAIKKIEAALA	acetylation	[1]
170	LTDAAIKKIEAALAD	acetylation	[1]
178	IEAALADKEAELMQF	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. 

Sequence Annotation

 MOD_RES 162 162 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 185 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:EcoCyc.
 GO:0043022; F:ribosome binding; IDA:EcoCyc.
 GO:0032790; P:ribosome disassembly; IMP:EcoCyc.
 GO:0006415; P:translational termination; IEA:HAMAP. 

Interpro

 IPR002661; Ribosome_recyc_fac.
 IPR023584; Ribosome_recyc_fac_dom. 

Pfam

 PF01765; RRF 

SMART

  

PROSITE

  

PRINTS