CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-015165
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tetratricopeptide repeat protein 27 
Protein Synonyms/Alias
 TPR repeat protein 27 
Gene Name
 TTC27 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
273DLTGALGKRTRFQENubiquitination[1]
313TPQEHLTKNLELNDDubiquitination[2, 3]
420LADQFEDKTTSVLERubiquitination[1, 2, 3]
484ERAGQHGKAEEILRQubiquitination[1, 3]
517GDHSCYDKAWELSRYubiquitination[3]
533SARAQRSKALLHLRNubiquitination[3]
541ALLHLRNKEFQECVEubiquitination[3]
691GDVATGLKGKLQELFubiquitination[1, 3]
693VATGLKGKLQELFGRubiquitination[2, 3, 4]
732EKPDENEKAFQCLSKubiquitination[2]
739KAFQCLSKAYKCDTQubiquitination[3]
742QCLSKAYKCDTQSNCubiquitination[2]
752TQSNCWEKDITSFKEacetylation[4]
758EKDITSFKEVVQRALubiquitination[3]
773GLAHVAIKCSKNKSSubiquitination[3]
778AIKCSKNKSSSQEAVubiquitination[3]
803RGLLSKAKQLFTDVAubiquitination[3, 5, 6, 7]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
  
Sequence Annotation
 REPEAT 440 466 TPR 1.
 REPEAT 467 499 TPR 2.
 REPEAT 527 560 TPR 3.
 REPEAT 541 573 HAT 1.
 REPEAT 561 594 TPR 4.
 REPEAT 596 628 TPR 5.
 REPEAT 606 638 HAT 2.
 REPEAT 629 662 TPR 6.
 REPEAT 689 721 HAT 3.
 REPEAT 731 767 TPR 7.  
Keyword
 Complete proteome; Polymorphism; Reference proteome; Repeat; TPR repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 843 AA 
Protein Sequence
MWTPELAILR GFPTEAERQQ WKQEGVVGSE SGSFLQLLLE GNYEAIFLNS MTQNIFNSTT 60
TAEEKIDSYL EKQVVTFLDY STDLDTTERQ QLIFLLGVSS LQLFVQSNWT GPPVDLHPQD 120
FLSSVLFQQF SEVKGLDAFV LSLLTLDGES IYSLTSKPIL LLLARIILVN VRHKLTAIQS 180
LPWWTLRCVN IHQHLLEERS PLLFTLAENC IDQVMKLQNL FVDDSGRYLA IQFHLECAYV 240
FLYYYEYRKA KDQLDIAKDI SQLQIDLTGA LGKRTRFQEN YVAQLILDVR REGDVLSNCE 300
FTPAPTPQEH LTKNLELNDD TILNDIKLAD CEQFQMPDLC AEEIAIILGI CTNFQKNNPV 360
HTLTEVELLA FTSCLLSQPK FWAIQTSALI LRTKLEKGST RRVERAMRQT QALADQFEDK 420
TTSVLERLKI FYCCQVPPHW AIQRQLASLL FELGCTSSAL QIFEKLEMWE DVVICYERAG 480
QHGKAEEILR QELEKKETPS LYCLLGDVLG DHSCYDKAWE LSRYRSARAQ RSKALLHLRN 540
KEFQECVECF ERSVKINPMQ LGVWFSLGCA YLALEDYQGS AKAFQRCVTL EPDNAEAWNN 600
LSTSYIRLKQ KVKAFRTLQE ALKCNYEHWQ IWENYILTST DVGEFSEAIK AYHRLLDLRD 660
KYKDVQVLKI LVRAVIDGMT DRSGDVATGL KGKLQELFGR VTSRVTNDGE IWRLYAHVYG 720
NGQSEKPDEN EKAFQCLSKA YKCDTQSNCW EKDITSFKEV VQRALGLAHV AIKCSKNKSS 780
SQEAVQMLSS VRLNLRGLLS KAKQLFTDVA TGEMSRELAD DITAMDTLVT ELQDLSNQFR 840
NQY 843 
Gene Ontology
  
Interpro
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS