CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000886
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sjoegren syndrome/scleroderma autoantigen 1 
Protein Synonyms/Alias
 Autoantigen p27 
Gene Name
 SSSCA1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22PPTEAETKVLQARREubiquitination[1, 2, 3, 4, 5]
64GTILLQDKQRKIYCVubiquitination[3, 4, 6, 7]
67LLQDKQRKIYCVACQubiquitination[4]
82ELDSDVDKDNPALNAubiquitination[6, 8]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983
Functional Description
 Might play a role in mitosis. Antigenic molecule. Could be a centromere-associated protein. May induce anti-centromere antibodies. 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 94 94 Phosphoserine.  
Keyword
 Acetylation; Cell cycle; Cell division; Complete proteome; Mitosis; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 199 AA 
Protein Sequence
MALNGAEVDD FSWEPPTEAE TKVLQARRER QDRISRLMGD YLLRGYRMLG ETCADCGTIL 60
LQDKQRKIYC VACQELDSDV DKDNPALNAQ AALSQAREHQ LASASELPLG SRPAPQPPVP 120
RPEHCEGAAA GLKAAQGPPA PAVPPNTDVM ACTQTALLQK LTWASAELGS STSLETSIQL 180
CGLIRACAEA LRSLQQLQH 199 
Gene Ontology
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; TAS:ProtInc. 
Interpro
 IPR009563; Unchr_Sjogrens_synd/UPF0148. 
Pfam
 PF06677; Auto_anti-p27 
SMART
  
PROSITE
  
PRINTS