CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016242
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ras GTPase-activating-like protein IQGAP3 
Protein Synonyms/Alias
  
Gene Name
 IQGAP3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
314QSPEALLKALQDPALubiquitination[1]
386HAVQRINKAIRRRVAubiquitination[2]
819RRLHYFQKNVNSIVKubiquitination[3]
938SDMMVLDKQKGLKSLacetylation[4]
1021LQEEIKSKVEQPQDVubiquitination[5]
1067IQDVLEDKVLSVHTDubiquitination[3]
1158LKATLAEKFPDATDSubiquitination[3]
1245YLEETHLKFRKFIHRubiquitination[3]
1402REQEAAHKQLMSRRQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 DOMAIN 34 149 CH.
 DOMAIN 730 759 IQ 1.
 DOMAIN 760 789 IQ 2.
 DOMAIN 790 819 IQ 3.
 DOMAIN 820 849 IQ 4.
 DOMAIN 988 1221 Ras-GAP.
 MOD_RES 539 539 Phosphoserine.
 MOD_RES 1424 1424 Phosphoserine.  
Keyword
 3D-structure; Calmodulin-binding; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1631 AA 
Protein Sequence
MERRAAGPGW AAYERLTAEE MDEQRRQNVA YQYLCRLEEA KRWMEACLKE ELPSPVELEE 60
SLRNGVLLAK LGHCFAPSVV PLKKIYDVEQ LRYQATGLHF RHTDNINFWL SAIAHIGLPS 120
TFFPETTDIY DKKNMPRVVY CIHALSLFLF RLGLAPQIHD LYGKVKFTAE ELSNMASELA 180
KYGLQLPAFS KIGGILANEL SVDEAAVHAA VLAINEAVER GVVEDTLAAL QNPSALLENL 240
REPLAAVYQE MLAQAKMEKA ANARNHDDRE SQDIYDHYLT QAEIQGNINH VNVHGALEVV 300
DDALERQSPE ALLKALQDPA LALRGVRRDF ADWYLEQLNS DREQKAQELG LVELLEKEEV 360
QAGVAAANTK GDQEQAMLHA VQRINKAIRR RVAADTVKEL MCPEAQLPPV YPVASSMYQL 420
ELAVLQQQQG ELGQEELFVA VEMLSAVVLI NRALEARDAS GFWSSLVNPA TGLAEVEGEN 480
AQRYFDALLK LRQERGMGED FLSWNDLQAT VSQVNAQTQE ETDRVLAVSL INEALDKGSP 540
EKTLSALLLP AAGLDDVSLP VAPRYHLLLV AAKRQKAQVT GDPGAVLWLE EIRQGVVRAN 600
QDTNTAQRMA LGVAAINQAI KEGKAAQTER VLRNPAVALR GVVPDCANGY QRALESAMAK 660
KQRPADTAFW VQHDMKDGTA YYFHLQTFQG IWEQPPGCPL NTSHLTREEI QSAVTKVTAA 720
YDRQQLWKAN VGFVIQLQAR LRGFLVRQKF AEHSHFLRTW LPAVIKIQAH WRGYRQRKIY 780
LEWLQYFKAN LDAIIKIQAW ARMWAARRQY LRRLHYFQKN VNSIVKIQAF FRARKAQDDY 840
RILVHAPHPP LSVVRRFAHL LNQSQQDFLA EAELLKLQEE VVRKIRSNQQ LEQDLNIMDI 900
KIGLLVKNRI TLQEVVSHCK KLTKRNKEQL SDMMVLDKQK GLKSLSKEKR QKLEAYQHLF 960
YLLQTQPIYL AKLIFQMPQN KTTKFMEAVI FSLYNYASSR REAYLLLQLF KTALQEEIKS 1020
KVEQPQDVVT GNPTVVRLVV RFYRNGRGQS ALQEILGKVI QDVLEDKVLS VHTDPVHLYK 1080
NWINQTEAQT GQRSHLPYDV TPEQALSHPE VQRRLDIALR NLLAMTDKFL LAITSSVDQI 1140
PYGMRYVAKV LKATLAEKFP DATDSEVYKV VGNLLYYRFL NPAVVAPDAF DIVAMAAGGA 1200
LAAPQRHALG AVAQLLQHAA AGKAFSGQSQ HLRVLNDYLE ETHLKFRKFI HRACQVPEPE 1260
ERFAVDEYSD MVAVAKPMVY ITVGELVNTH RLLLEHQDCI APDHQDPLHE LLEDLGELPT 1320
IPDLIGESIA ADGHTDLSKL EVSLTLTNKF EGLEADADDS NTRSLLLSTK QLLADIIQFH 1380
PGDTLKEILS LSASREQEAA HKQLMSRRQA CTAQTPEPLR RHRSLTAHSL LPLAEKQRRV 1440
LRNLRRLEAL GLVSARNGYQ GLVDELAKDI RNQHRHRHRR KAELVKLQAT LQGLSTKTTF 1500
YEEQGDYYSQ YIRACLDHLA PDSKSSGKGK KQPSLHYTAA QLLEKGVLVE IEDLPASHFR 1560
NVIFDITPGD EAGKFEVNAK FLGVDMERFQ LHYQDLLQLQ YEGVAVMKLF NKAKVNVNLL 1620
IFLLNKKFLR K 1631 
Gene Ontology
 GO:0005911; C:cell-cell junction; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0005099; F:Ras GTPase activator activity; IEA:InterPro.
 GO:0000187; P:activation of MAPK activity; IEA:Compara.
 GO:0071310; P:cellular response to organic substance; IEA:Compara.
 GO:0070371; P:ERK1 and ERK2 cascade; IEA:Compara.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IEA:Compara.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0033601; P:positive regulation of mammary gland epithelial cell proliferation; IEA:Compara.
 GO:0032320; P:positive regulation of Ras GTPase activity; IEA:GOC.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:0008361; P:regulation of cell size; IEA:Compara. 
Interpro
 IPR001715; CH-domain.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR027417; P-loop_NTPase.
 IPR001936; RasGAP.
 IPR000593; RasGAP_C.
 IPR023152; RasGAP_CS.
 IPR008936; Rho_GTPase_activation_prot. 
Pfam
 PF00307; CH
 PF00612; IQ
 PF00616; RasGAP
 PF03836; RasGAP_C 
SMART
 SM00033; CH
 SM00015; IQ
 SM00323; RasGAP 
PROSITE
 PS50021; CH
 PS50096; IQ
 PS00509; RAS_GTPASE_ACTIV_1
 PS50018; RAS_GTPASE_ACTIV_2 
PRINTS