UniProt Accession

 KPYK2_ECOLI; P21599 

Genbank Protein ID

 M63703; U00096; AP009048; M77039; M87660 

Genbank Nucleotide ID

 AAA24473.1; AAC74924.1; BAA15662.1; AAA96707.1 

Protein Name

 Pyruvate kinase II 

Protein Synonyms/Alias

 PK-2 

Gene Name

 pykA 

Gene Synonyms/Alias

 b1854; JW1843 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
26	DRDNNLEKVIAAGAN	acetylation	[1, 2]
48	HGSPEDHKMRADKVR	acetylation	[2]
60	KVREIAAKLGRHVAI	acetylation	[2]
60	KVREIAAKLGRHVAI	pupylation	[3]
75	LGDLQGPKIRVSTFK	acetylation	[1, 2]
82	KIRVSTFKEGKVFLN	acetylation	[1, 2]
85	VSTFKEGKVFLNIGD	acetylation	[2]
93	VFLNIGDKFLLDANL	acetylation	[2]
93	VFLNIGDKFLLDANL	pupylation	[3]
102	LLDANLGKGEGDKEK	acetylation	[1, 2]
107	LGKGEGDKEKVGIDY	acetylation	[2]
115	EKVGIDYKGLPADVV	acetylation	[2]
137	DDGRVQLKVLEVQGM	acetylation	[1, 2]
160	GGPLSNNKGINKLGG	acetylation	[2]
177	SAEALTEKDKADIKT	acetylation	[2]
179	EALTEKDKADIKTAA	acetylation	[2]
343	RVCLGAEKIPSINVS	acetylation	[1, 2]
351	IPSINVSKHRLDVQF	acetylation	[1, 2, 4]

Reference

 [1] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [2] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [3] Reconstitution of the Mycobacterium tuberculosis pupylation pathway in Escherichia coli.
 Cerda-Maira FA, McAllister F, Bode NJ, Burns KE, Gygi SP, Darwin KH.
 EMBO Rep. 2011 Jul 8;12(8):863-70. [PMID: 21738222]
 [4] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

  

Sequence Annotation

 METAL 38 38 Potassium (By similarity).
 METAL 40 40 Potassium (By similarity).
 METAL 70 70 Potassium (By similarity).
 METAL 225 225 Magnesium (By similarity).
 METAL 252 252 Magnesium (By similarity).
 BINDING 36 36 Substrate (By similarity).
 BINDING 251 251 Substrate; via amide nitrogen (By
 BINDING 252 252 Substrate; via amide nitrogen (By
 BINDING 284 284 Substrate (By similarity).
 MOD_RES 351 351 N6-acetyllysine.  

Keyword

 Acetylation; Allosteric enzyme; ATP-binding; Complete proteome; Direct protein sequencing; Glycolysis; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Potassium; Pyruvate; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 480 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030955; F:potassium ion binding; IEA:InterPro.
 GO:0004743; F:pyruvate kinase activity; IEA:EC.
 GO:0006096; P:glycolysis; IEA:UniProtKB-UniPathway. 

Interpro

 IPR001697; Pyr_Knase.
 IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
 IPR011037; Pyrv_Knase-like_insert_dom.
 IPR015794; Pyrv_Knase_a/b.
 IPR018209; Pyrv_Knase_AS.
 IPR015793; Pyrv_Knase_brl.
 IPR015795; Pyrv_Knase_C.
 IPR015806; Pyrv_Knase_insert_dom. 

Pfam

 PF00224; PK
 PF02887; PK_C 

SMART

  

PROSITE

 PS00110; PYRUVATE_KINASE 

PRINTS

 PR01050; PYRUVTKNASE.