UniProt Accession

 RS4A_YEAST; P0CX35; D3DLF2; P05753 

Genbank Protein ID

 M64293; Z49645; BK006943 

Genbank Nucleotide ID

 AAA35011.1; CAA89678.1; DAA08930.1 

Protein Name

 40S ribosomal protein S4-A 

Protein Synonyms/Alias

 RP5; S7; YS6 

Gene Name

 RPS4A 

Gene Synonyms/Alias

 RPS7B; YJR145C; J2186 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
22	PHHWLLDKLSGCYAP	ubiquitination	[1]
37	RPSAGPHKLRESLPL	ubiquitination	[1]
53	VFLRNRLKYALNGRE	acetylation	[2]
53	VFLRNRLKYALNGRE	ubiquitination	[1]
62	ALNGREVKAILMQRH	ubiquitination	[1]
106	FRLVYDVKGRFAVHR	acetylation	[2]
106	FRLVYDVKGRFAVHR	ubiquitination	[1]
122	TDEEASYKLGKVKKV	acetylation	[2]
122	TDEEASYKLGKVKKV	ubiquitination	[1]
134	KKVQLGKKGVPYVVT	ubiquitination	[1]
155	RYPDPNIKVNDTVKI	ubiquitination	[1]
161	IKVNDTVKIDLASGK	acetylation	[2]
161	IKVNDTVKIDLASGK	ubiquitination	[1]
168	KIDLASGKITDFIKF	acetylation	[2]
168	KIDLASGKITDFIKF	ubiquitination	[1, 3]
174	GKITDFIKFDAGKLV	acetylation	[2]
174	GKITDFIKFDAGKLV	ubiquitination	[1]
179	FIKFDAGKLVYVTGG	acetylation	[2]
179	FIKFDAGKLVYVTGG	ubiquitination	[1]
198	RIGTIVHKERHDGGF	acetylation	[2]
198	RIGTIVHKERHDGGF	ubiquitination	[1]
211	GFDLVHIKDSLDNTF	ubiquitination	[1]
233	FVIGEQGKPYISLPK	acetylation	[2]
233	FVIGEQGKPYISLPK	ubiquitination	[1]
240	KPYISLPKGKGIKLS	ubiquitination	[1]
245	LPKGKGIKLSIAEER	acetylation	[2]
245	LPKGKGIKLSIAEER	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080] 

Functional Description

  

Sequence Annotation

 DOMAIN 42 105 S4 RNA-binding.
 MOD_RES 32 32 Phosphoserine.
 MOD_RES 115 115 Phosphothreonine.
 MOD_RES 247 247 Phosphoserine.
 CROSSLNK 168 168 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Phosphoprotein; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0030686; C:90S preribosome; IDA:SGD.
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
 GO:0003735; F:structural constituent of ribosome; IDA:SGD.
 GO:0002181; P:cytoplasmic translation; IC:SGD. 

Interpro

 IPR005824; KOW.
 IPR000876; Ribosomal_S4e.
 IPR013845; Ribosomal_S4e_central_region.
 IPR013843; Ribosomal_S4e_N.
 IPR018199; Ribosomal_S4e_N_CS.
 IPR002942; S4_RNA-bd. 

Pfam

 PF00467; KOW
 PF00900; Ribosomal_S4e
 PF08071; RS4NT
 PF01479; S4 

SMART

 SM00739; KOW
 SM00363; S4 

PROSITE

 PS00528; RIBOSOMAL_S4E
 PS50889; S4 

PRINTS