UniProt Accession

 F5H425_HUMAN; F5H425 

Genbank Protein ID

 AC009996 

Genbank Nucleotide ID

  

Protein Name

 Eukaryotic translation initiation factor 3 subunit J 

Protein Synonyms/Alias

 eIF3j; Eukaryotic translation initiation factor 3 subunit 1; eIF-3-alpha; eIF3 p35 

Gene Name

 EIF3J 

Gene Synonyms/Alias

 EIF3S1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
112	DDFTEFGKLLKDKIT	ubiquitination	[1, 2, 3]
117	FGKLLKDKITQYEKS	ubiquitination	[2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA (By similarity). 

Sequence Annotation

 REGION 1 44 Sufficient for interaction with EIF3B (By
 REGION 194 209 Promotes stable association with the 40S
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 11 11 Phosphoserine (By similarity).
 MOD_RES 13 13 Phosphoserine (By similarity).
 MOD_RES 20 20 Phosphoserine (By similarity).
 MOD_RES 60 60 Phosphothreonine (By similarity).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 209 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IEA:HAMAP.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 

Interpro

 IPR023194; eIF3-like_dom.
 IPR013906; eIF3j. 

Pfam

 PF08597; eIF3_subunit 

SMART

  

PROSITE

  

PRINTS