UniProt Accession

 ALKB2_HUMAN; Q6NS38; A4PET2; Q5XLE3 

Genbank Protein ID

 AY754389; AB277859; AC011596; BC070489 

Genbank Nucleotide ID

 AAV28301.1; BAF56576.1; AAH70489.1 

Protein Name

 Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 

Protein Synonyms/Alias

 Alkylated DNA repair protein alkB homolog 2; DNA oxidative demethylase ALKBH2; Oxy DC1 

Gene Name

 ALKBH2 

Gene Synonyms/Alias

 ABH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
139	GIRIPVGKAPPGGWR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. 

Sequence Annotation

 DOMAIN 152 257 Fe2OG dioxygenase.
 REGION 102 104 Substrate binding.
 REGION 122 124 Substrate binding.
 REGION 159 161 Alpha-ketoglutarate binding.
 REGION 248 254 Alpha-ketoglutarate binding.
 METAL 171 171 Iron; catalytic.
 METAL 173 173 Iron; catalytic.
 METAL 236 236 Iron; catalytic.
 BINDING 174 174 Substrate.  

Keyword

 3D-structure; Alternative splicing; Complete proteome; Dioxygenase; DNA damage; DNA repair; Iron; Magnesium; Metal-binding; Nucleus; Oxidoreductase; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0051747; F:cytosine C-5 DNA demethylase activity; IDA:UniProtKB.
 GO:0043734; F:DNA-N1-methyladenine dioxygenase activity; IDA:UniProtKB.
 GO:0008198; F:ferrous iron binding; IDA:UniProtKB.
 GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:UniProtKB-KW.
 GO:0006307; P:DNA dealkylation involved in DNA repair; IDA:UniProtKB.
 GO:0035511; P:oxidative DNA demethylation; IDA:UniProtKB. 

Interpro

 IPR027450; AlkB-like.
 IPR005123; Oxoglu/Fe-dep_dioxygenase. 

Pfam

 PF13532; 2OG-FeII_Oxy_2 

SMART

  

PROSITE

 PS51471; FE2OG_OXY 

PRINTS