UniProt Accession

 DPOE3_HUMAN; Q9NRF9; Q5W0U1; Q8N758; Q8NCE5; Q9NR32 

Genbank Protein ID

 AF226077; AF261689; AY720898; AK074629; AK074762; AK074782; DQ072116; AL137066; BC003166; BC004170 

Genbank Nucleotide ID

 AAF72417.1; AAF90133.1; AAU15052.1; BAC11099.1; BAC11190.1; BAC11206.1; AAY57326.1; CAH70100.1; AAH03166.1; AAH04170.1 

Protein Name

 DNA polymerase epsilon subunit 3 

Protein Synonyms/Alias

 Arsenic-transactivated protein; AsTP; Chromatin accessibility complex 17 kDa protein; CHRAC-17; HuCHRAC17; DNA polymerase II subunit 3; DNA polymerase epsilon subunit p17 

Gene Name

 POLE3 

Gene Synonyms/Alias

 CHRAC17 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
20	AVITRIIKEALPDGV	ubiquitination	[1, 2]
31	PDGVNISKEARSAIS	ubiquitination	[1, 2, 3, 4, 5]
62	FAMKGKRKTLNASDV	ubiquitination	[1]
86	QRFVTPLKEALEAYR	ubiquitination	[1, 3, 5, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 83 83 Phosphothreonine.
 MOD_RES 122 122 Phosphoserine.  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; DNA-binding; DNA-directed DNA polymerase; Nucleotidyltransferase; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 147 AA 

Protein Sequence

Gene Ontology

 GO:0005671; C:Ada2/Gcn5/Ada3 transcription activator complex; IDA:BHF-UCL.
 GO:0008622; C:epsilon DNA polymerase complex; IDA:UniProtKB.
 GO:0003887; F:DNA-directed DNA polymerase activity; TAS:ProtInc.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0006260; P:DNA replication; TAS:ProtInc.
 GO:0043966; P:histone H3 acetylation; IDA:BHF-UCL. 

Interpro

 IPR003958; CBFA_NFYB_domain.
 IPR009072; Histone-fold. 

Pfam

 PF00808; CBFD_NFYB_HMF 

SMART

  

PROSITE

  

PRINTS