UniProt Accession

 ARPC4_HUMAN; P59998; C9JWM7; E7ETI0; F6TTL5; O15509; Q6P0W5; Q96QJ3 

Genbank Protein ID

 AF019888; AF006087; BX419672; AC022382; BC012596; BC025289 

Genbank Nucleotide ID

 AAB71548.1; AAB64192.1; AAH12596.3 

Protein Name

 Actin-related protein 2/3 complex subunit 4 

Protein Synonyms/Alias

 Arp2/3 complex 20 kDa subunit; p20-ARC 

Gene Name

 ARPC4 

Gene Synonyms/Alias

 ARC20 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
35	QVVERHNKPEVEVRS	acetylation	[1]
44	EVEVRSSKELLLQPV	ubiquitination	[2, 3, 4, 5]
89	IEKILCHKFMRFMMM	acetylation	[1]
89	IEKILCHKFMRFMMM	ubiquitination	[4]
166	IVAEEFLKNF*****	ubiquitination	[4, 6, 7, 8]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [8] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Functions as actin-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the mother actin filament. 

Sequence Annotation

 MOD_RES 2 2 N-acetylthreonine.  

Keyword

 Acetylation; Actin-binding; Alternative splicing; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 168 AA 

Protein Sequence

Gene Ontology

 GO:0005885; C:Arp2/3 protein complex; IDA:UniProtKB.
 GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030041; P:actin filament polymerization; IEA:InterPro.
 GO:0045010; P:actin nucleation; NAS:UniProtKB.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome. 

Interpro

 IPR008384; ARPC4. 

Pfam

 PF05856; ARPC4 

SMART

  

PROSITE

  

PRINTS