UniProt Accession

 MED10_HUMAN; Q9BTT4; C6G491 

Genbank Protein ID

 AY598325; AY277599; AY453397; FJ515848; CH471102; BC003353 

Genbank Nucleotide ID

 AAT06736.1; AAQ18038.1; AAS47514.1; ACS13739.1; EAX08110.1; AAH03353.1 

Protein Name

 Mediator of RNA polymerase II transcription subunit 10 

Protein Synonyms/Alias

 Mediator complex subunit 10; Transformation-related gene 17 protein; TRG-17; Transformation-related gene 20 protein; TRG-20 

Gene Name

 MED10 

Gene Synonyms/Alias

 L6; TRG17; TRG20 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
4	****MAEKFDHLEEH	ubiquitination	[1, 2, 3, 4]
14	HLEEHLEKFVENIRQ	ubiquitination	[2]
53	TGLQDIDKCRQQLHD	ubiquitination	[2]
82	RNPQLYTKECLERAL	ubiquitination	[4, 5]
106	IDTMKKFKSLLIQEL	ubiquitination	[2]
115	LLIQELSKVFPEDMA	ubiquitination	[1, 2, 3, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. 

Sequence Annotation

  

Keyword

 Activator; Complete proteome; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 135 AA 

Protein Sequence

Gene Ontology

 GO:0016592; C:mediator complex; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IEA:InterPro.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0019827; P:stem cell maintenance; IEA:Compara.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; TAS:Reactome. 

Interpro

 IPR019145; Mediator_Med10. 

Pfam

 PF09748; Med10 

SMART

  

PROSITE

  

PRINTS