CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000730
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Regulator of G-protein signaling 14 
Protein Synonyms/Alias
 RGS14 
Gene Name
 RGS14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
98AENVTFWKACERFQQubiquitination[1]
176DSYARFVKSPLYRECubiquitination[1]
287SKSESHRKSLGSTEGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. 
Sequence Annotation
 DOMAIN 67 184 RGS.
 DOMAIN 302 373 RBD 1.
 DOMAIN 375 445 RBD 2.
 DOMAIN 498 521 GoLoco.
 REGION 299 425 Necessary for interaction with RABGEF1
 MOD_RES 20 20 Phosphoserine.
 MOD_RES 42 42 Phosphoserine.
 MOD_RES 45 45 Phosphoserine.
 MOD_RES 288 288 Phosphoserine (By similarity).  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Cell junction; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; GTPase activation; Membrane; Microtubule; Nucleus; Phosphoprotein; Postsynaptic cell membrane; Reference proteome; Repeat; Signal transduction inhibitor; Synapse. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 566 AA 
Protein Sequence
MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP 60
VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN 120
IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY 180
RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL 240
RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP 300
GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLP DIKVYLVGNE QALVLDQDCT 360
VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG 420
EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS 480
SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLL RKEDLVLPEF LQLPAQGPSS 540
EETPPQTKSA AQPIGGSLNS TTDSAL 566 
Gene Ontology
 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0045111; C:intermediate filament cytoskeleton; IDA:HPA.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0016604; C:nuclear body; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; ISS:UniProtKB.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0014069; C:postsynaptic density; ISS:UniProtKB.
 GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-KW.
 GO:0000922; C:spindle pole; ISS:UniProtKB.
 GO:0005092; F:GDP-dissociation inhibitor activity; IDA:UniProtKB.
 GO:0005096; F:GTPase activator activity; IDA:UniProtKB.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0030159; F:receptor signaling complex scaffold activity; ISS:UniProtKB.
 GO:0005057; F:receptor signaling protein activity; ISS:UniProtKB.
 GO:0007059; P:chromosome segregation; ISS:UniProtKB.
 GO:0007616; P:long-term memory; ISS:UniProtKB.
 GO:0060291; P:long-term synaptic potentiation; ISS:UniProtKB.
 GO:0007067; P:mitosis; IEA:Compara.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; ISS:UniProtKB.
 GO:0043407; P:negative regulation of MAP kinase activity; ISS:UniProtKB.
 GO:0031914; P:negative regulation of synaptic plasticity; ISS:UniProtKB.
 GO:0006913; P:nucleocytoplasmic transport; ISS:UniProtKB.
 GO:0048008; P:platelet-derived growth factor receptor signaling pathway; ISS:UniProtKB.
 GO:0050769; P:positive regulation of neurogenesis; ISS:UniProtKB.
 GO:0043620; P:regulation of DNA-dependent transcription in response to stress; ISS:UniProtKB.
 GO:0008277; P:regulation of G-protein coupled receptor protein signaling pathway; TAS:ProtInc.
 GO:0006979; P:response to oxidative stress; ISS:UniProtKB.
 GO:0007051; P:spindle organization; IMP:UniProtKB.
 GO:0038032; P:termination of G-protein coupled receptor signaling pathway; IEA:InterPro.
 GO:0008542; P:visual learning; ISS:UniProtKB.
 GO:0010070; P:zygote asymmetric cell division; ISS:UniProtKB. 
Interpro
 IPR003109; GoLoco_motif.
 IPR003116; Raf-like_ras-bd.
 IPR000342; Regulat_G_prot_signal.
 IPR024066; Regulat_G_prot_signal_dom1.
 IPR016137; Regulat_G_prot_signal_superfam. 
Pfam
 PF02188; GoLoco
 PF02196; RBD
 PF00615; RGS 
SMART
 SM00390; GoLoco
 SM00455; RBD
 SM00315; RGS 
PROSITE
 PS50877; GOLOCO
 PS50898; RBD
 PS50132; RGS 
PRINTS
 PR01301; RGSPROTEIN.