UniProt Accession

 PLST_HUMAN; P13797; A8K579; B1AQ09; Q86YI6 

Genbank Protein ID

 M22299; M34427; AK291194; AK312391; AL589842; AC005000; CH471120; BC039049; BC056898; L05491 

Genbank Nucleotide ID

 AAB02844.1; AAA36759.1; BAF83883.1; BAG35308.1; CAI39884.1; CAI39884.1; EAX02614.1; AAH39049.1; AAH56898.1; AAA61214.1 

Protein Name

 Plastin-3 

Protein Synonyms/Alias

 T-plastin 

Gene Name

 PLS3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
52	NMPLPGYKVREIIQK	ubiquitination	[1, 2]
59	KVREIIQKLMLDGDR	ubiquitination	[1]
91	VKSSDIAKTFRKAIN	ubiquitination	[1, 2]
100	FRKAINRKEGICALG	ubiquitination	[1]
168	GDGIVLCKMINLSVP	ubiquitination	[1, 3, 4]
266	ETLEELMKLSPEELL	ubiquitination	[2, 4]
297	NNFSADIKDSKAYFH	ubiquitination	[2]
300	SADIKDSKAYFHLLN	ubiquitination	[1, 2]
382	NKYPALTKPENQDID	ubiquitination	[1, 2]
437	LQLYERIKVPVDWSK	ubiquitination	[1]
518	EDLGDGQKANDDIIV	ubiquitination	[2]
545	STSIQSFKDKTISSS	ubiquitination	[2]
587	DDKHNNAKYAVSMAR	ubiquitination	[2]
611	PEDLVEVKPKMVMTV	ubiquitination	[1, 2, 4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry.
 Denis NJ, Vasilescu J, Lambert JP, Smith JC, Figeys D.
 Proteomics. 2007 Mar;7(6):868-74. [PMID: 17370265]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. 

Sequence Annotation

 DOMAIN 12 47 EF-hand 1.
 DOMAIN 52 87 EF-hand 2.
 DOMAIN 109 382 Actin-binding 1.
 DOMAIN 123 239 CH 1.
 DOMAIN 267 378 CH 2.
 DOMAIN 383 627 Actin-binding 2.
 DOMAIN 397 506 CH 3.
 DOMAIN 518 627 CH 4.
 MOD_RES 293 293 Phosphoserine.
 MOD_RES 326 326 Phosphoserine.
 MOD_RES 391 391 Phosphothreonine.
 CROSSLNK 168 168 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Actin-binding; Calcium; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Metal-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 630 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro. 

Interpro

 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 

Pfam

 PF00307; CH
 PF13499; EF_hand_5 

SMART

 SM00033; CH
 SM00054; EFh 

PROSITE

 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS