CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025269
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heat shock 70 kDa protein 4 
Protein Synonyms/Alias
 Heat shock protein 4, isoform CRA_a 
Gene Name
 Hspa4 
Gene Synonyms/Alias
 mCG_113604 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
44ACVSFGPKNRSIGAAubiquitination[1]
53RSIGAAAKSQVISNAacetylation[2]
53RSIGAAAKSQVISNAsuccinylation[2]
61SQVISNAKNTVQGFKubiquitination[1]
124VTAMLLSKLKETAESacetylation[3]
124VTAMLLSKLKETAESubiquitination[1]
126AMLLSKLKETAESVLubiquitination[1]
135TAESVLKKPVVDCVVubiquitination[1]
194DLPALEEKPRNVVFVubiquitination[1]
235DTTLGGRKFDEVLVNacetylation[3]
235DTTLGGRKFDEVLVNubiquitination[1]
272RLSQECEKLKKLMSAacetylation[3]
305SGTMNRGKFLEMCDDacetylation[3]
305SGTMNRGKFLEMCDDubiquitination[1]
329RSVLEQSKLKKEDIYubiquitination[1]
430NHAAPFSKVLTFYRKubiquitination[1]
575QAKKAKVKTSTVDLPubiquitination[1]
605LYTENEGKMIMQDKLubiquitination[1]
620EKERNDAKNAVEEYVubiquitination[1]
633YVYEMRDKLSGEYEKubiquitination[1]
640KLSGEYEKFVSEDDRubiquitination[1]
670EDGEDQPKQVYVDKLacetylation[4]
676PKQVYVDKLAELKSLacetylation[4]
676PKQVYVDKLAELKSLubiquitination[1]
681VDKLAELKSLGQPIKacetylation[3]
681VDKLAELKSLGQPIKubiquitination[1]
688KSLGQPIKTRFQESEubiquitination[1]
706KLFEELGKQIQQYMKubiquitination[1]
713KQIQQYMKVISSFKNubiquitination[1]
719MKVISSFKNKEDQYEubiquitination[1]
721VISSFKNKEDQYEHLubiquitination[1]
756SKLNLQNKQSLTVDPubiquitination[1]
775KEIEAKIKELTSICSacetylation[3]
775KEIEAKIKELTSICSubiquitination[1]
787ICSPIISKPKPKVEPacetylation[3]
787ICSPIISKPKPKVEPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Nucleotide-binding; Reference proteome; Stress response. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 842 AA 
Protein Sequence
MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSIGA AAKSQVISNA 60
KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM 120
LLSKLKETAE SVLKKPVVDC VVSVPSFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA 180
YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNKGKL KVLATAFDTT LGGRKFDEVL 240
VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT 300
MNRGKFLEMC DDLLARVEPP LRSVLEQSKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK 360
ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGLSDCEVF 420
PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV 480
KVRVNVHGIF SVSSAALVEV HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQQQPQT 540
PAENKAESEE METSQAGSKD KKTDQPPQAK KAKVKTSTVD LPIESQLLWQ LDREMLGLYT 600
ENEGKMIMQD KLEKERNDAK NAVEEYVYEM RDKLSGEYEK FVSEDDRNTF TLKLEDTENW 660
LYEDGEDQPK QVYVDKLAEL KSLGQPIKTR FQESEERPKL FEELGKQIQQ YMKVISSFKN 720
KEDQYEHLDA ADVTKVEKST NEAMEWMNSK LNLQNKQSLT VDPVVKTKEI EAKIKELTSI 780
CSPIISKPKP KVEPPKEEPK HAEQNGPVDG QGDNPGSQAA EHGADTAVPS DGDKKLPEMD 840
ID 842 
Gene Ontology
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0051131; P:chaperone-mediated protein complex assembly; IEA:Compara.
 GO:0045040; P:protein import into mitochondrial outer membrane; IEA:Compara.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 
Interpro
 IPR018181; Heat_shock_70_CS.
 IPR013126; Hsp_70_fam. 
Pfam
 PF00012; HSP70 
SMART
  
PROSITE
 PS00329; HSP70_2
 PS01036; HSP70_3 
PRINTS
 PR00301; HEATSHOCK70.