UniProt Accession

 HAND2_MOUSE; Q61039; Q61100 

Genbank Protein ID

 U40039; AK035160; U43715 

Genbank Nucleotide ID

 AAC52338.1; BAC28965.1; AAA86274.1 

Protein Name

 Heart- and neural crest derivatives-expressed protein 2 

Protein Synonyms/Alias

 Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2; dHAND; Helix-loop-helix transcription factor expressed in embryo and deciduum; Thing-2 

Gene Name

 Hand2 

Gene Synonyms/Alias

 Dhand; Hed; Thing2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
183	VKEEKRKKELNEILK	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'. 

Sequence Annotation

 DOMAIN 99 151 bHLH.  

Keyword

 Angiogenesis; Complete proteome; Developmental protein; Differentiation; DNA-binding; Nucleus; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 217 AA 

Protein Sequence

Gene Ontology

 GO:0000790; C:nuclear chromatin; IEA:Compara.
 GO:0005667; C:transcription factor complex; IDA:BHF-UCL.
 GO:0033613; F:activating transcription factor binding; ISS:BHF-UCL.
 GO:0003680; F:AT DNA binding; IEA:Compara.
 GO:0070888; F:E-box binding; IDA:MGI.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IEA:Compara.
 GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IGI:MGI.
 GO:0003713; F:transcription coactivator activity; ISS:BHF-UCL.
 GO:0007512; P:adult heart development; IMP:MGI.
 GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
 GO:0003278; P:apoptotic process involved in heart morphogenesis; IMP:MGI.
 GO:0003253; P:cardiac neural crest cell migration involved in outflow tract morphogenesis; IMP:MGI.
 GO:0003219; P:cardiac right ventricle formation; IMP:BHF-UCL.
 GO:0060536; P:cartilage morphogenesis; IGI:MGI.
 GO:0061325; P:cell proliferation involved in outflow tract morphogenesis; IMP:MGI.
 GO:0060982; P:coronary artery morphogenesis; IMP:MGI.
 GO:0042733; P:embryonic digit morphogenesis; IDA:MGI.
 GO:0001947; P:heart looping; IMP:MGI.
 GO:0001701; P:in utero embryonic development; IMP:MGI.
 GO:0010463; P:mesenchymal cell proliferation; IMP:MGI.
 GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; IMP:BHF-UCL.
 GO:0043392; P:negative regulation of DNA binding; IDA:MGI.
 GO:0045668; P:negative regulation of osteoblast differentiation; IDA:MGI.
 GO:0043433; P:negative regulation of sequence-specific DNA binding transcription factor activity; IDA:MGI.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IGI:MGI.
 GO:0060021; P:palate development; IMP:MGI.
 GO:0048935; P:peripheral nervous system neuron development; IDA:MGI.
 GO:2000764; P:positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis; IMP:BHF-UCL.
 GO:2000763; P:positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process; IMP:BHF-UCL.
 GO:2000679; P:positive regulation of transcription regulatory region DNA binding; IEA:Compara.
 GO:0003266; P:regulation of secondary heart field cardioblast proliferation; IMP:BHF-UCL.
 GO:0001967; P:suckling behavior; IMP:MGI.
 GO:0048485; P:sympathetic nervous system development; IDA:MGI.
 GO:0048538; P:thymus development; IMP:BHF-UCL.
 GO:0043586; P:tongue development; IMP:MGI.
 GO:0061032; P:visceral serous pericardium development; IMP:MGI. 

Interpro

 IPR011598; bHLH_dom. 

Pfam

 PF00010; HLH 

SMART

 SM00353; HLH 

PROSITE

 PS50888; BHLH 

PRINTS