CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024606
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-Ig 
Protein Synonyms/Alias
 Minor histocompatibility antigen HA-2; mHag HA-2 
Gene Name
 MYO1G 
Gene Synonyms/Alias
 HA2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87AVANAAYKAMKHRSRubiquitination[1, 2, 3]
108SGESGAGKTEASKHIubiquitination[2]
113AGKTEASKHIMQYIAubiquitination[2]
135RAEVERVKDVLLKSTubiquitination[2]
140RVKDVLLKSTCVLEAubiquitination[2]
164HNSSRFGKYMDINFDubiquitination[2]
188IHSYLLEKSRVLKQHubiquitination[4]
193LEKSRVLKQHVGERNubiquitination[1, 2]
214LLRGSEDKQLHELHLubiquitination[2, 3]
341GGRELIEKGHTAAEAubiquitination[1, 2, 3]
357YARDACAKAVYQRLFubiquitination[1, 2]
507RQLCPTDKTMEFGRDacetylation[3]
507RQLCPTDKTMEFGRDubiquitination[1, 2, 3]
518FGRDFRIKHYAGDVTmethylation[5]
518FGRDFRIKHYAGDVTubiquitination[2]
534SVEGFIDKNRDFLFQubiquitination[2]
544DFLFQDFKRLLYNSTubiquitination[1, 2, 4, 6]
571QDITEVTKRPLTAGTubiquitination[1, 2, 3, 4]
581LTAGTLFKNSMVALVubiquitination[2]
594LVENLASKEPFYVRCubiquitination[1, 2, 3]
603PFYVRCIKPNEDKVAubiquitination[2]
608CIKPNEDKVAGKLDEacetylation[3]
608CIKPNEDKVAGKLDEubiquitination[2, 3]
612NEDKVAGKLDENHCRubiquitination[2, 3]
652SRFLLRYKMTCEYTWubiquitination[2, 3]
690DVAFGHSKLFIRSPRubiquitination[2, 3]
802WRARQLVKNIPPSDMubiquitination[2]
815DMPQIKAKVAAMGALubiquitination[1, 2, 3, 4]
863RLKTLQDKDGFGAVLubiquitination[4]
898LTDQHLYKLDPDRQYubiquitination[1, 2, 3, 4]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity). 
Sequence Annotation
 DOMAIN 13 683 Myosin head-like.
 DOMAIN 710 739 IQ.
 NP_BIND 102 109 ATP (By similarity).  
Keyword
 Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding; Cell membrane; Complete proteome; Direct protein sequencing; Membrane; Motor protein; Myosin; Nucleotide-binding; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1018 AA 
Protein Sequence
MEDEEGPEYG KPDFVLLDQV TMEDFMRNLQ LRFEKGRIYT YIGEVLVSVN PYQELPLYGP 60
EAIARYQGRE LYERPPHLYA VANAAYKAMK HRSRDTCIVI SGESGAGKTE ASKHIMQYIA 120
AVTNPSQRAE VERVKDVLLK STCVLEAFGN ARTNRNHNSS RFGKYMDINF DFKGDPIGGH 180
IHSYLLEKSR VLKQHVGERN FHAFYQLLRG SEDKQLHELH LERNPAVYNF THQGAGLNMT 240
VHSALDSDEQ SHQAVTEAMR VIGFSPEEVE SVHRILAAIL HLGNIEFVET EEGGLQKEGL 300
AVAEEALVDH VAELTATPRD LVLRSLLART VASGGRELIE KGHTAAEASY ARDACAKAVY 360
QRLFEWVVNR INSVMEPRGR DPRRDGKDTV IGVLDIYGFE VFPVNSFEQF CINYCNEKLQ 420
QLFIQLILKQ EQEEYEREGI TWQSVEYFNN ATIVDLVERP HRGILAVLDE ACSSAGTITD 480
RIFLQTLDMH HRHHLHYTSR QLCPTDKTME FGRDFRIKHY AGDVTYSVEG FIDKNRDFLF 540
QDFKRLLYNS TDPTLRAMWP DGQQDITEVT KRPLTAGTLF KNSMVALVEN LASKEPFYVR 600
CIKPNEDKVA GKLDENHCRH QVAYLGLLEN VRVRRAGFAS RQPYSRFLLR YKMTCEYTWP 660
NHLLGSDKAA VSALLEQHGL QGDVAFGHSK LFIRSPRTLV TLEQSRARLI PIIVLLLQKA 720
WRGTLARWRC RRLRAIYTIM RWFRRHKVRA HLAELQRRFQ AARQPPLYGR DLVWPLPPAV 780
LQPFQDTCHA LFCRWRARQL VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL 840
SSATDNPTAS SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD 900
PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL DNRVGELVGV 960
LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP EPDFRCARGS FTLLWPSR 1018 
Gene Ontology
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003774; F:motor activity; IEA:InterPro. 
Interpro
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR010926; Myosin_tail_2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00063; Myosin_head
 PF06017; Myosin_TH1 
SMART
 SM00015; IQ
 SM00242; MYSc 
PROSITE
 PS50096; IQ 
PRINTS
 PR00193; MYOSINHEAVY.