CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013709
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Enolase 
Protein Synonyms/Alias
 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase 
Gene Name
 eno 
Gene Synonyms/Alias
 GK3054 
Created Date
 July 27, 2013 
Organism
 Geobacillus kaustophilus (strain HTA426) 
NCBI Taxa ID
 235909 
Lysine Modification
Position
Peptide
Type
References
103ELDGTENKSKLGANAacetylation[1]
232AIEKAGYKPGEQVMLacetylation[1]
308LTERLGKKVQLVGDDacetylation[1]
322DLFVTNTKKLAEGIEacetylation[1]
Reference
 [1] Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus.
 Lee DW, Kim D, Lee YJ, Kim JA, Choi JY, Kang S, Pan JG.
 Proteomics. 2013 Aug;13(15):2278-82. [PMID: 23696451] 
Functional Description
 Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis (By similarity). 
Sequence Annotation
 REGION 366 369 Substrate binding (By similarity).
 ACT_SITE 205 205 Proton donor (By similarity).
 ACT_SITE 339 339 Proton acceptor (By similarity).
 METAL 242 242 Magnesium (By similarity).
 METAL 287 287 Magnesium (By similarity).
 METAL 314 314 Magnesium (By similarity).
 BINDING 155 155 Substrate (By similarity).
 BINDING 164 164 Substrate (By similarity).
 BINDING 287 287 Substrate (By similarity).
 BINDING 314 314 Substrate (By similarity).
 BINDING 339 339 Substrate (covalent); in inhibited form
 BINDING 390 390 Substrate (By similarity).
 MOD_RES 141 141 Phosphothreonine (By similarity).
 MOD_RES 281 281 Phosphotyrosine (By similarity).  
Keyword
 Complete proteome; Cytoplasm; Glycolysis; Lyase; Magnesium; Metal-binding; Phosphoprotein; Secreted. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 430 AA 
Protein Sequence
MSAIIDVYAR EVLDSRGNPT VEVEVYTEDG GFGRALVPSG ASTGEYEAVE LRDGDKNRYL 60
GKGVLKAVEN VNEIIAPEII GLEVADQVAI DRKLIELDGT ENKSKLGANA ILGVSLAVAR 120
AAADELGLPL YQYLGGFNAK TLPVPMMNIL NGGAHADNNV DIQEFMIMPV GAESFREALR 180
MGAEIFHSLK AVLKAKGYNT AVGDEGGFAP NLKSNEEALQ TIIEAIEKAG YKPGEQVMLA 240
MDVASSELYN KEDGKYHLEG EGVVKTSEEM VAWYEELVSK YPIISIEDGL DENDWEGHKL 300
LTERLGKKVQ LVGDDLFVTN TKKLAEGIEK GVGNSILIKV NQIGTLTETF DAIEMAKRAG 360
YTAVVSHRSG ETEDSTIADI AVATNAGQIK TGAPSRTDRV AKYNQLLRIE DELGHTAIYQ 420
GIRSFYNLKK 430 
Gene Ontology
 GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0004634; F:phosphopyruvate hydratase activity; IEA:HAMAP.
 GO:0006096; P:glycolysis; IEA:HAMAP. 
Interpro
 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 
Pfam
 PF00113; Enolase_C
 PF03952; Enolase_N 
SMART
  
PROSITE
 PS00164; ENOLASE 
PRINTS
 PR00148; ENOLASE.