CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014151
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Threonine--tRNA ligase, cytoplasmic 
Protein Synonyms/Alias
 Threonyl-tRNA synthetase; ThrRS 
Gene Name
 Tars 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
35TRLDMYHKLKAEHDSacetylation[1]
521PKIDIQIKDAIGRYHacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405
Functional Description
  
Sequence Annotation
 MOD_RES 215 215 N6-acetyllysine (By similarity).
 MOD_RES 270 270 Phosphotyrosine (By similarity).
 CROSSLNK 194 194 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Nucleotide-binding; Phosphoprotein; Protein biosynthesis; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 695 AA 
Protein Sequence
MSEEKASSPS GKMDGEKPLN PWPEYINTRL DMYHKLKAEH DSILAEKAAK DSKPIKVTLP 60
DGKQVDAESW KTTPYQIACG ISQGLADNTV VAKVNKVVWD LDRPLETDCT LELLKFEDEE 120
AQAVYWHSSA HIMGEAMERV YGGCLCYGPP IENGFYYDMY LEEGGVSSND FSSLETLCKK 180
IIKEKQTFER LEVKKETLLE MFKYNKFKCR ILNEKVNTPT TTVYRCGPLI DLCRGPHVRH 240
TGKIKTLKIH KNSSTYWEGK ADMETLQRIY GISFPDPKLL KEWEKFQEEA KNRDHRKIGR 300
DQELYFFHEL SPGSCFFLPK GAYIYNTLME FIRSEYRKRG FQEVVTPNIF NSRLWMTSGH 360
WQHYSENMFS FEVEKEQFAL KPMNCPGHCL MFDHRPRSWR ELPLRLADFG VLHRNELSGA 420
LTGLTRVRRF QQDDAHIFCA MEQIEDEIKG CLDFLRTVYS VFGFSFKLNL STRPEKFLGD 480
IEIWNQAEKQ LENSLNEFGE KWELNPGDGA FYGPKIDIQI KDAIGRYHQC ATIQLDFQLP 540
IRFNLTYVSH DGDDKKRPVI VHRAILGSVE RMIAILTENY GGKWPFWLSP RQVMVVPVGP 600
TCDEYAQKVR QEFHDAKFMV DIDLDPGCTL NKKIRNAQLA QYNFILVVGE KEKASGTVNI 660
RTRDNKVHGE RTVGETVERL QQLKQLRSKQ AEEEF 695 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IEA:Compara.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004829; F:threonine-tRNA ligase activity; IEA:EC.
 GO:0006435; P:threonyl-tRNA aminoacylation; IEA:InterPro. 
Interpro
 IPR002314; aa-tRNA-synt_IIb_cons-dom.
 IPR006195; aa-tRNA-synth_II.
 IPR004154; Anticodon-bd.
 IPR012675; Beta-grasp_dom.
 IPR004095; TGS.
 IPR012676; TGS-like.
 IPR002320; Thr-tRNA-ligase_IIa.
 IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
 IPR012947; tRNA_SAD. 
Pfam
 PF03129; HGTP_anticodon
 PF02824; TGS
 PF00587; tRNA-synt_2b
 PF07973; tRNA_SAD 
SMART
 SM00863; tRNA_SAD 
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR01047; TRNASYNTHTHR.