| Tag | Content |
|---|
| CPLM ID | CPLM-007876 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | SPS-sensor serine protease component SSY5 |
Protein Synonyms/Alias | Endoprotease SSY5 |
Gene Name | SSY5 |
Gene Synonyms/Alias | APF8; YJL156C; J0570 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 108 | HSGSKNSKNLQGFKD | ubiquitination | [1] | | 210 | IEAIEYFKEFLPTTR | ubiquitination | [1] | | 228 | PYRISLEKSSSLRKI | ubiquitination | [1] | | 566 | NTLGNGLKSFPDPTL | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Protease component of the SPS-sensor system, which regulates the expression of several amino acid-metabolizing enzymes and amino acid- and peptide-permeases in response to extracellular amino acid levels by controlling the activity of two transcription factors, STP1 and STP2. Catalyzes the activation of these transcription factors, which are synthesized as latent cytoplasmic precursors, by proteolytic removal of an N-terminal inhibitory domain containing cytoplasmic retention motifs. SSY5 binds as an inactive protease complex to STP1. In response to extracellular amino acids and dependent on the other SPS-sensor components, the inhibitory propeptide is induced to dissociate, and thereby enables the catalytic domain to process STP1. |
Sequence Annotation | REGION 459 699 Serine protease.
ACT_SITE 465 465 Charge relay system (Potential).
ACT_SITE 545 545 Charge relay system (Potential).
ACT_SITE 640 640 Charge relay system (Potential). |
Keyword | Autocatalytic cleavage; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Membrane; Protease; Reference proteome; Zymogen. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 699 AA |
Protein Sequence | MVRFFGLNKK KNEEKENTDL PADNEQNAAE TSSSNVSGNE ERIDPNSHDT NPENANNDDA 60
STTFGSSIQS SSIFSRGRMT YGTGASSSMA TSEMRSHSSG HSGSKNSKNL QGFKDVGKPL 120
RAVSFLSPVK EEESQDTQNT LDVSSSTSST LATSENAREN SFTSRRSITL EYIHKSLSEL 180
EENLVDIMDD IHQDVISISK AVIEAIEYFK EFLPTTRDRI PYRISLEKSS SLRKINKIVL 240
HFLDNLLVSD AFSNSRSILL RRFYFFLKKL NLITDDDLIS ESGVLPCLSV FCIGSHCNLP 300
SMDKLGMILD ELTKMDSSII SDQEGAFIAP ILRGITPKSS ILTIMFGLPN LQHEHYEMIK 360
VLYSLFPDVH MYCVKDYIKK AASAVGSIPS HTAATIDTIA PTKFQFSPPY AVSENPLELP 420
ISMSLSTETS AKITGTLGGY LFPQTGSDKK FSQFASCSFA ITCAHVVLSE KQDYPNVMVP 480
SNVLQTSYKK VLTKESDRYP DGSVEKTAFL EEVQRIDQNL NWQKSNKFGQ VVWGERAIVD 540
HRLSDFAIIK VNSSFKCQNT LGNGLKSFPD PTLRFQNLHV KRKIFKMKPG MKVFKIGAST 600
GYTSGELNST KLVYWADGKL QSSEFVVASP TPLFASAGDS GAWILTKLED RLGLGLVGML 660
HSYDGEQRQF GLFTPIGDIL ERLHAVTKIQ WDIDPQLDG 699 |
Gene Ontology | GO:0019897; C:extrinsic to plasma membrane; IDA:SGD. GO:0004252; F:serine-type endopeptidase activity; IDA:SGD. GO:0016485; P:protein processing; IDA:SGD. GO:0006508; P:proteolysis; IEA:UniProtKB-KW. GO:0043200; P:response to amino acid stimulus; IMP:SGD. |
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Pfam | |
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PRINTS | |