CPLM-015133

Genbank Nucleotide ID

Polyamine-modulated factor 1

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
16	LGSGCEEKRHEGSSS	ubiquitination	[1, 2]
36	GTTISRVKLLDTMVD	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
48	MVDTFLQKLVAAGSY	ubiquitination	[4]
63	QRFTDCYKCFYQLQP	ubiquitination	[1]
96	REEISDIKEEGNLEA	ubiquitination	[4]
116	DKIVEEGKVRKEPAW	ubiquitination	[1]
119	VEEGKVRKEPAWRPS	ubiquitination	[5]
131	RPSGIPEKDLHSVMA	ubiquitination	[1]
154	TLRRHVQKQEAENQQ	ubiquitination	[1, 2, 4, 5]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[5] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[7] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]

Functional Description

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.

MOD_RES 2 2 N-acetylalanine.

Acetylation; Activator; Alternative splicing; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Coiled coil; Complete proteome; Kinetochore; Mitosis; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005829; C:cytosol; TAS:Reactome.
GO:0000444; C:MIS12/MIND type complex; IDA:UniProtKB.
GO:0005667; C:transcription factor complex; TAS:ProtInc.
GO:0003713; F:transcription coactivator activity; IMP:UniProtKB.
GO:0051301; P:cell division; IEA:UniProtKB-KW.
GO:0007059; P:chromosome segregation; IMP:UniProtKB.
GO:0007067; P:mitosis; IEA:UniProtKB-KW.
GO:0000278; P:mitotic cell cycle; TAS:Reactome.
GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc.