| Tag | Content |
|---|
| CPLM ID | CPLM-006578 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Protein DOA1 |
Protein Synonyms/Alias | |
Gene Name | DOA1 |
Gene Synonyms/Alias | UFD3; ZZZ4; YKL213C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 460 | SSAVSPSKTSVMKVL | ubiquitination | [1] | | 465 | PSKTSVMKVLPVKQY | ubiquitination | [1] |
|
Reference | [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation. Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J. Nat Methods. 2013 Jul;10(7):676-82. [ PMID: 23749301] |
Functional Description | Participates in the regulation of the ubiquitin conjugation pathway involving CDC48 by hindering multiubiquitination of substrates at the CDC48 chaperone. May act by preventing the interaction between CDC48 and the E4 enzyme UFD2, leading to prevent multiubiquitination of substrates and subsequent degradation. Essential for maintaining cellular ubiquitin levels. |
Sequence Annotation | REPEAT 11 40 WD 1.
REPEAT 53 82 WD 2.
REPEAT 97 125 WD 3.
REPEAT 135 166 WD 4.
REPEAT 177 206 WD 5.
REPEAT 218 247 WD 6.
REPEAT 259 288 WD 7.
DOMAIN 352 449 PFU.
DOMAIN 465 715 PUL.
REPEAT 478 512 ARM 1.
REPEAT 513 543 ARM 2.
REPEAT 544 582 ARM 3.
REPEAT 583 635 ARM 4.
REPEAT 636 680 ARM 5.
REPEAT 681 715 ARM 6.
MOD_RES 332 332 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 715 AA |
Protein Sequence | MGYQLSATLK GHDQDVRDVV AVDDSKVASV SRDGTVRLWS KDDQWLGTVV YTGQGFLNSV 60
CYDSEKELLL FGGKDTMING VPLFATSGED PLYTLIGHQG NVCSLSFQDG VVISGSWDKT 120
AKVWKEGSLV YNLQAHNASV WDAKVVSFSE NKFLTASADK TIKLWQNDKV IKTFSGIHND 180
VVRHLAVVDD GHFISCSNDG LIKLVDMHTG DVLRTYEGHE SFVYCIKLLP NGDIVSCGED 240
RTVRIWSKEN GSLKQVITLP AISIWSVDCM SNGDIIVGSS DNLVRIFSQE KSRWASEDEI 300
NELSTQVEKS TISSKTIEFD ESKLSPYEIL QSPGRKEGQI VVVKSPQGTI EAHQFSNSSW 360
KKVGDVVGAG ATGNDKKIEF EGKTYDYVFD VDIEDGKPPL KLPINVSDNP YTAADNFLAR 420
YELPMSYRDQ VVQFILKNTN GISLDQPNDN ASSSAVSPSK TSVMKVLPVK QYLIMENYNP 480
DTIFNGIVKI NSNEKTFDDE ILAQIGGALH DIDESWELLL SFANTIRSNW EIKTPAYDIV 540
RLIVKKLPYS SDIKDYIEEG LGNKNITLTM LTVRILVNCF NNENWGVKLL ESNQVYKSIF 600
ETIDTEFSQA SAKQSQNLAI AVSTLIFNYS ALVTKGNSDL ELLPIVADAI NTKYGPLEEY 660
QECEEAAYRL TVAYGNLATV EPTLRQFANS VTWLANIKRS YGNVPRFKDI FDDLS 715 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0043130; F:ubiquitin binding; IDA:SGD. GO:0006303; P:double-strand break repair via nonhomologous end joining; IMP:SGD. GO:0006511; P:ubiquitin-dependent protein catabolic process; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |