| Tag | Content |
|---|
| CPLM ID | CPLM-010969 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Nucleoporin NUP116/NSP116 |
Protein Synonyms/Alias | Nuclear pore protein NUP116/NSP116 |
Gene Name | NUP116 |
Gene Synonyms/Alias | NSP116; YMR047C; YM9532.12C |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 796 | PKTLFTAKLKTNNSV | acetylation | [1] |
|
Reference | [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases. Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K. Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [ PMID: 23572591] |
Functional Description | Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP116 plays an important role in several nuclear export and import pathways including poly(A)+ RNA, tRNA, pre- ribosome, and protein transport. |
Sequence Annotation | REPEAT 2 3 FG 1.
REPEAT 17 18 FG 2.
REPEAT 24 25 FG 3.
REPEAT 40 41 FG 4.
REPEAT 55 58 GLFG 1; approximate.
REPEAT 66 67 FG 5.
REPEAT 79 80 FG 6.
REPEAT 94 95 FG 7.
REPEAT 167 168 FG 8.
REPEAT 189 190 FG 9.
REPEAT 205 208 GLFG 2.
REPEAT 214 217 GLFG 3; approximate.
REPEAT 224 227 GLFG 4; approximate.
REPEAT 235 238 GLFG 5.
REPEAT 249 250 FG 10.
REPEAT 259 262 GLFG 6.
REPEAT 276 279 GLFG 7.
REPEAT 288 291 GLFG 8.
REPEAT 297 298 FG 11.
REPEAT 306 309 GLFG 9; approximate.
REPEAT 327 330 GLFG 10; approximate.
REPEAT 339 342 GLFG 11; approximate.
REPEAT 351 352 FG 12.
REPEAT 359 362 GLFG 12.
REPEAT 370 371 FG 13.
REPEAT 382 385 GLFG 13.
REPEAT 395 398 GLFG 14.
REPEAT 407 410 GLFG 15.
REPEAT 420 423 GLFG 16.
REPEAT 431 432 FG 14.
REPEAT 439 442 GLFG 17.
REPEAT 448 451 GLFG 18.
REPEAT 470 471 FG 15.
REPEAT 482 485 GLFG 19.
REPEAT 497 500 GLFG 20.
REPEAT 510 511 FG 16.
REPEAT 525 526 FG 17.
REPEAT 532 533 FG 18.
REPEAT 572 575 GLFG 21.
REPEAT 585 588 GLFG 22.
REPEAT 604 607 GLFG 23.
REPEAT 616 617 FG 19.
REPEAT 630 633 GLFG 24; approximate.
REPEAT 648 651 GLFG 25.
REPEAT 665 668 GLFG 26.
REPEAT 683 686 GLFG 27.
DOMAIN 967 1109 Peptidase S59.
REGION 110 166 GLE2 binding sequence (GLEBS).
REGION 160 362 Interaction with MEX67, not KAP95.
REGION 362 535 Sufficient for interaction with MEX67 and
REGION 536 732 Interaction with KAP95, not MEX67.
REGION 967 1113 Interaction with NUP82 NPC subcomplex.
REGION 969 1108 Nucleoporin RNA-binding motif (NRM).
MOD_RES 886 886 Phosphoserine. |
Keyword | 3D-structure; Complete proteome; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Translocation; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 1113 AA |
Protein Sequence | MFGVSRGAFP SATTQPFGST GSTFGGQQQQ QQPVANTSAF GLSQQTNTTQ APAFGNFGNQ 60
TSNSPFGMSG STTANGTPFG QSQLTNNNAS GSIFGGMGNN TALSAGSASV VPNSTAGTSI 120
KPFTTFEEKD PTTGVINVFQ SITCMPEYRN FSFEELRFQD YQAGRKFGTS QNGTGTTFNN 180
PQGTTNTGFG IMGNNNSTTS ATTGGLFGQK PATGMFGTGT GSGGGFGSGA TNSTGLFGSS 240
TNLSGNSAFG ANKPATSGGL FGNTTNNPTN GTNNTGLFGQ QNSNTNGGLF GQQQNSFGAN 300
NVSNGGAFGQ VNRGAFPQQQ TQQGSGGIFG QSNANANGGA FGQQQGTGAL FGAKPASGGL 360
FGQSAGSKAF GMNTNPTGTT GGLFGQTNQQ QSGGGLFGQQ QNSNAGGLFG QNNQSQNQSG 420
LFGQQNSSNA FGQPQQQGGL FGSKPAGGLF GQQQGASTFA SGNAQNNSIF GQNNQQQQST 480
GGLFGQQNNQ SQSQPGGLFG QTNQNNNQPF GQNGLQQPQQ NNSLFGAKPT GFGNTSLFSN 540
STTNQSNGIS GNNLQQQSGG LFQNKQQPAS GGLFGSKPSN TVGGGLFGNN QVANQNNPAS 600
TSGGLFGSKP ATGSLFGGTN STAPNASSGG IFGSNNASNT AATTNSTGLF GNKPVGAGAS 660
TSAGGLFGNN NNSSLNNSNG STGLFGSNNT SQSTNAGGLF QNNTSTNTSG GGLFSQPSQS 720
MAQSQNALQQ QQQQQRLQIQ NNNPYGTNEL FSKATVTNTV SYPIQPSATK IKADERKKAS 780
LTNAYKMIPK TLFTAKLKTN NSVMDKAQIK VDPKLSISID KKNNQIAISN QQEENLDESI 840
LKASELLFNP DKRSFKNLIN NRKMLIASEE KNNGSQNNDM NFKSKSEEQE TILGKPKMDE 900
KETANGGERM VLSSKNDGED SATKHHSRNM DEENKENVAD LQKQEYSEDD KKAVFADVAE 960
KDASFINENY YISPSLDTLS SYSLLQLRKV PHLVVGHKSY GKIEFLEPVD LAGIPLTSLG 1020
GVIITFEPKT CIIYANLPNR PKRGEGINVR ARITCFNCYP VDKSTRKPIK DPNHQLVKRH 1080
IERLKKNPNS KFESYDADSG TYVFIVNHAA EQT 1113 |
Gene Ontology | GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell. GO:0044613; C:nuclear pore central transport channel; IDA:SGD. GO:0044614; C:nuclear pore cytoplasmic filaments; IDA:SGD. GO:0005487; F:nucleocytoplasmic transporter activity; IGI:SGD. GO:0017056; F:structural constituent of nuclear pore; IMP:SGD. GO:0006999; P:nuclear pore organization; IMP:SGD. GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:SGD. GO:0006606; P:protein import into nucleus; IMP:SGD. GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD. GO:0006409; P:tRNA export from nucleus; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
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PRINTS | |