UniProt Accession

 ENOA_RAT; P04764; Q66HI3; Q6AYV3; Q6P504 

Genbank Protein ID

 X02610; BC063174; BC078896; BC081847; AF241613 

Genbank Nucleotide ID

 CAA26456.1; AAH63174.1; AAH78896.1; AAH81847.2; AAK01319.1 

Protein Name

 Alpha-enolase 

Protein Synonyms/Alias

 2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE 

Gene Name

 Eno1 

Gene Synonyms/Alias

 Eno-1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
64	FMGKGVSKAVEHINK	ubiquitination	[1]
81	APALVSKKLNVVEQE	ubiquitination	[1]
202	VIKEKYGKDATNVGD	ubiquitination	[1]
330	TNPKRIAKAAGEKSC	ubiquitination	[1]
420	IEEELGSKAKFAGRS	ubiquitination	[1]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. 

Sequence Annotation

 REGION 370 373 Substrate binding (By similarity).
 REGION 405 434 Required for interaction with PLG.
 ACT_SITE 210 210 Proton donor (By similarity).
 ACT_SITE 343 343 Proton acceptor (By similarity).
 METAL 245 245 Magnesium (By similarity).
 METAL 293 293 Magnesium (By similarity).
 METAL 318 318 Magnesium (By similarity).
 BINDING 158 158 Substrate (By similarity).
 BINDING 167 167 Substrate (By similarity).
 BINDING 293 293 Substrate (By similarity).
 BINDING 318 318 Substrate (By similarity).
 BINDING 394 394 Substrate (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 5 5 N6-acetyllysine (By similarity).
 MOD_RES 44 44 Phosphotyrosine (By similarity).
 MOD_RES 64 64 N6-acetyllysine (By similarity).
 MOD_RES 71 71 N6-acetyllysine (By similarity).
 MOD_RES 89 89 N6-acetyllysine (By similarity).
 MOD_RES 126 126 N6-acetyllysine (By similarity).
 MOD_RES 193 193 N6-acetyllysine (By similarity).
 MOD_RES 199 199 N6-acetyllysine (By similarity).
 MOD_RES 228 228 N6-acetyllysine (By similarity).
 MOD_RES 233 233 N6-acetyllysine; alternate (By
 MOD_RES 233 233 N6-malonyllysine; alternate (By
 MOD_RES 256 256 N6-acetyllysine (By similarity).
 MOD_RES 263 263 Phosphoserine (By similarity).
 MOD_RES 281 281 N6-acetyllysine (By similarity).
 MOD_RES 285 285 N6-acetyllysine (By similarity).
 MOD_RES 287 287 Phosphotyrosine (By similarity).
 MOD_RES 420 420 N6-acetyllysine; alternate (By
 MOD_RES 420 420 N6-malonyllysine; alternate (By  

Keyword

 Acetylation; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Glycolysis; Lyase; Magnesium; Membrane; Metal-binding; Phosphoprotein; Plasminogen activation; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 434 AA 

Protein Sequence

Gene Ontology

 GO:0043005; C:neuron projection; IDA:RGD.
 GO:0000015; C:phosphopyruvate hydratase complex; IEA:InterPro.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0097060; C:synaptic membrane; IDA:RGD.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0004634; F:phosphopyruvate hydratase activity; IDA:RGD.
 GO:0046982; F:protein heterodimerization activity; IDA:RGD.
 GO:0042803; F:protein homodimerization activity; IDA:RGD.
 GO:0006096; P:glycolysis; TAS:RGD. 

Interpro

 IPR000941; Enolase.
 IPR020810; Enolase_C.
 IPR020809; Enolase_CS.
 IPR020811; Enolase_N. 

Pfam

 PF00113; Enolase_C
 PF03952; Enolase_N 

SMART

  

PROSITE

 PS00164; ENOLASE 

PRINTS

 PR00148; ENOLASE.