CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011656
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 [NU+] prion formation protein 1 
Protein Synonyms/Alias
  
Gene Name
 NEW1 
Gene Synonyms/Alias
 YPL226W; P1445 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
45AGSNNASKKSSYQQQacetylation[1]
101YNGQGYQKSTYKQSAubiquitination[2]
158CQSITDCKNQIKLIIubiquitination[2]
190KIVDVLSKFIKPKNPacetylation[3]
322TDVATDFKPELAKQGacetylation[3]
546DSQREFVKQDFIHNLacetylation[3]
628QLDGFPDKDTLRTCFubiquitination[2]
916DDREVLLKESRKISEacetylation[3]
990GFEKLVQKFDDHEASacetylation[3]
1119SAQVDQSKFEDGGNAubiquitination[2]
1138LKASNLAKPSVDDDDacetylation[3]
1138LKASNLAKPSVDDDDubiquitination[2]
1183IEWLSSPKGTPKPVDubiquitination[2]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 May be involved in the mRNA export process (By similarity). Forms the [NU+] prion and induces [PSI+] prion formation. 
Sequence Annotation
 DOMAIN 570 786 ABC transporter 1.
 DOMAIN 812 1129 ABC transporter 2.
 DOMAIN 942 1003 Chromo.
 NP_BIND 604 611 ATP 1 (Potential).
 NP_BIND 846 853 ATP 2 (Potential).
 MOD_RES 443 443 Phosphoserine.
 MOD_RES 1191 1191 Phosphothreonine.  
Keyword
 Amyloid; ATP-binding; Complete proteome; Cytoplasm; mRNA transport; Nucleotide-binding; Nucleus; Phosphoprotein; Prion; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1196 AA 
Protein Sequence
MPPKKFKDLN SFLDDQPKDP NLVASPFGGY FKNPAADAGS NNASKKSSYQ QQRNWKQGGN 60
YQQGGYQSYN SNYNNYNNYN NYNNYNNYNN YNKYNGQGYQ KSTYKQSAVT PNQSGTPTPS 120
ASTTSLTSLN EKLSNLELTP ISQFLSKIPE CQSITDCKNQ IKLIIEEFGK EGNSTGEKIE 180
EWKIVDVLSK FIKPKNPSLV RESAMLIISN IAQFFSGKPP QEAYLLPFFN VALDCISDKE 240
NTVKRAAQHA IDSLLNCFPM EALTCFVLPT ILDYLSSGAK WQAKMAALSV VDRIREDSAN 300
DLLELTFKDA VPVLTDVATD FKPELAKQGY KTLLDYVSIL DNLDLSPRYK LIVDTLQDPS 360
KVPESVKSLS SVTFVAEVTE PSLSLLVPIL NRSLNLSSSS QEQLRQTVIV VENLTRLVNN 420
RNEIESFIPL LLPGIQKVVD TASLPEVREL AEKALNVLKE DDEADKENKF SGRLTLEEGR 480
DFLLDHLKDI KADDSCFVKP YMNDETVIKY MSKILTVDSN VNDWKRLEDF LTAVFGGSDS 540
QREFVKQDFI HNLRALFYQE KERADEDEGI EIVNTDFSLA YGSRMLLNKT NLRLLKGHRY 600
GLCGRNGAGK STLMRAIANG QLDGFPDKDT LRTCFVEHKL QGEEGDLDLV SFIALDEELQ 660
STSREEIAAA LESVGFDEER RAQTVGSLSG GWKMKLELAR AMLQKADILL LDEPTNHLDV 720
SNVKWLEEYL LEHTDITSLI VSHDSGFLDT VCTDIIHYEN KKLAYYKGNL AAFVEQKPEA 780
KSYYTLTDSN AQMRFPPPGI LTGVKSNTRA VAKMTDVTFS YPGAQKPSLS HVSCSLSLSS 840
RVACLGPNGA GKSTLIKLLT GELVPNEGKV EKHPNLRIGY IAQHALQHVN EHKEKTANQY 900
LQWRYQFGDD REVLLKESRK ISEDEKEMMT KEIDIDDGRG KRAIEAIVGR QKLKKSFQYE 960
VKWKYWKPKY NSWVPKDVLV EHGFEKLVQK FDDHEASREG LGYRELIPSV ITKHFEDVGL 1020
DSEIANHTPL GSLSGGQLVK VVIAGAMWNN PHLLVLDEPT NYLDRDSLGA LAVAIRDWSG 1080
GVVMISHNNE FVGALCPEQW IVENGKMVQK GSAQVDQSKF EDGGNADAVG LKASNLAKPS 1140
VDDDDSPANI KVKQRKKRLT RNEKKLQAER RRLRYIEWLS SPKGTPKPVD TDDEED 1196 
Gene Ontology
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005844; C:polysome; IDA:SGD.
 GO:0005524; F:ATP binding; ISS:SGD.
 GO:0016887; F:ATPase activity; ISS:SGD.
 GO:0016973; P:poly(A)+ mRNA export from nucleus; ISS:SGD.
 GO:0042274; P:ribosomal small subunit biogenesis; IMP:SGD. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR023780; Chromo_domain.
 IPR000953; Chromo_domain/shadow.
 IPR015688; Elongation_fac_3.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00005; ABC_tran
 PF00385; Chromo 
SMART
 SM00382; AAA
 SM00298; CHROMO 
PROSITE
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2
 PS00598; CHROMO_1
 PS50013; CHROMO_2 
PRINTS