CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004163
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translationally-controlled tumor protein 
Protein Synonyms/Alias
 TCTP; Fortilin; Histamine-releasing factor; HRF; p23 
Gene Name
 TPT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19EMFSDIYKIREIADGacetylation[1]
19EMFSDIYKIREIADGubiquitination[2, 3, 4, 5, 6, 7]
93EAYKKYIKDYMKSIKacetylation[8]
93EAYKKYIKDYMKSIKubiquitination[5]
97KYIKDYMKSIKGKLEacetylation[8]
97KYIKDYMKSIKGKLEubiquitination[5]
102YMKSIKGKLEEQRPEubiquitination[5, 9]
112EQRPERVKPFMTGAAacetylation[8]
112EQRPERVKPFMTGAAubiquitination[5, 6, 10]
123TGAAEQIKHILANFKubiquitination[5]
164TPYMIFFKDGLEMEKsumoylation[11]
164TPYMIFFKDGLEMEKubiquitination[5, 10]
171KDGLEMEKC******ubiquitination[5]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [9] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [10] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [11] Sumoylation of human translationally controlled tumor protein is important for its nuclear transport.
 Munirathinam G, Ramaswamy K.
 Biochem Res Int. 2012;2012:831940. [PMID: 22567286
Functional Description
 Involved in calcium binding and microtubule stabilization. 
Sequence Annotation
 MOD_RES 46 46 Phosphoserine; by PLK1.
 MOD_RES 53 53 Phosphoserine.
 MOD_RES 64 64 Phosphoserine; by PLK1.  
Keyword
 3D-structure; Calcium; Complete proteome; Cytoplasm; Direct protein sequencing; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 172 AA 
Protein Sequence
MIIYRDLISH DEMFSDIYKI REIADGLCLE VEGKMVSRTE GNIDDSLIGG NASAEGPEGE 60
GTESTVITGV DIVMNHHLQE TSFTKEAYKK YIKDYMKSIK GKLEEQRPER VKPFMTGAAE 120
QIKHILANFK NYQFFIGENM NPDGMVALLD YREDGVTPYM IFFKDGLEME KC 172 
Gene Ontology
 GO:0005615; C:extracellular space; IDA:MGI.
 GO:0005771; C:multivesicular body; IDA:MGI.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0006816; P:calcium ion transport; IC:UniProtKB.
 GO:0006874; P:cellular calcium ion homeostasis; IC:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
 GO:2000384; P:negative regulation of ectoderm development; IEA:Compara.
 GO:0009615; P:response to virus; IEP:UniProtKB.
 GO:0019827; P:stem cell maintenance; IEA:Compara. 
Interpro
 IPR011057; Mss4-like.
 IPR011323; Mss4/transl-control_tumor.
 IPR018103; Translation_control_tumour_CS.
 IPR018105; Translational_control_tumour_p. 
Pfam
 PF00838; TCTP 
SMART
  
PROSITE
 PS01002; TCTP_1
 PS01003; TCTP_2 
PRINTS
 PR01653; TCTPROTEIN.