CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014677
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Wings apart-like protein homolog 
Protein Synonyms/Alias
 Dioxin-inducible factor 2; DIF-2 
Gene Name
 Wapal 
Gene Synonyms/Alias
 Kiaa0261; Wapl 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
321QGASNFDKLMDGTSQubiquitination[1]
633QEIVTALKCRKEDKEubiquitination[1]
767EQDASSAKLLNEKDMubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister- chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair (By similarity). 
Sequence Annotation
 DOMAIN 635 1179 WAPL.
 REGION 1 668 Mediates interaction with the cohesin
 MOTIF 73 75 FGF motif 1.
 MOTIF 437 439 FGF motif 2.
 MOTIF 462 464 FGF motif 3.
 MOD_RES 77 77 Phosphoserine.
 MOD_RES 168 168 N6-acetyllysine (By similarity).
 MOD_RES 226 226 Phosphoserine.
 MOD_RES 228 228 Phosphoserine (By similarity).
 MOD_RES 388 388 Phosphoserine (By similarity).
 MOD_RES 468 468 Phosphoserine.
 MOD_RES 470 470 Phosphoserine.
 MOD_RES 914 914 Phosphoserine (By similarity).  
Keyword
 Acetylation; Cell cycle; Cell division; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Mitosis; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1200 AA 
Protein Sequence
MTSRFGKTYS RKGGNGSSKF DEVFSNKRTT LSTKWGETTF MAKLGQKRPN FKPDIQEIPK 60
KPKVEEEDTG DPFGFDSDDE SLPVSSKNLA QGKGSSYSES SEAAQLEEVT SVFEANSKCS 120
HVVGEDSFAS DRCLLVEDTL IGKEKSISRI PEDNANKSSC TKLLTSDKVE NFSEEHEKNS 180
HHFHKNAEDS TKKPNAETAV ASEYKADETK ETNDTWNSQS GKRTESPSES CPVKGSVRTG 240
LYEWDNDFED IRSEDCILSL DNESLLEMKD EDLKNRIGGL ENLNETFEED IIQSVLRPSN 300
CRTYCRANKA RSSQGASNFD KLMDGTSQSL AKANSESSKD GLNQAKKGSA SCGTSFRGTV 360
GRTRDYTVLH PSCLSVCNVT IQDTMERSMD EFTASTPADL GEAGRLRKKA DIATSKTTTR 420
FRPSNTKSKK DVKLEFFGFE DHDETGGDEG GSGSSNYKIK YFGFDDLSES EDDDDDDCQV 480
ERKKDKKRTK TAPSPSQQPP PESSDNSQDS QSSTNNAENL DFTEDLPGVP ESVKKPISKQ 540
GDKSKENTRK IFSGPKRSPT KAVYNARHWN HPDSEELPGP PIAKPQRVTV RLSSKEPNQK 600
DDGVFKAPAP PLKVIKTVTI PTQPYQEIVT ALKCRKEDKE LYTVVQHVKH FNDVVEFGEN 660
QEFTDDIEYL LSGLKSTQPL NTRCLSVISL ATKCAMPSFR MHLRAHGMVA MVFKTLDDSQ 720
HHQNLSLCTA ALMYILSRDR LNMDLDRASL DLMIRLLELE QDASSAKLLN EKDMNKIKEK 780
IRRLCETVHN KHLDLENITT GHLAMETLLS LTSKRAGDWF KEELRLLGGL DHIVDKVKEC 840
VDHLSRDDED EEKLVASLWG AERCLRVLES VTVHNPENQS YLIAYKDSQL IISSAKALQH 900
CEDLIQQYNR AENSICVADS NPLPYQNVTN HVGKAVEDCM RAIIGVLLNL TNDNEWGSTK 960
TGEQEGLIGT AMNCVLQVPK YLPQEQRFDI RVLGLGLLIN LVEYSARNRH CLVNMQTSCS 1020
FDSSFSSGEG DHSLRLAGQV HAVQALVQLF LERERAAQLA ESKTDELIKD APTTQHDKSG 1080
EWQETSGEIQ WVSTEKTDGA EEKQKKEEED EELDLNKALQ HAGKHMEDCI VASYTALLLG 1140
CLCQESPINV TTVREYLPEG DFSIMTEMLK KFLSFMNLTC AVGTTGQKSI SRVIEYLEHC 1200 
Gene Ontology
 GO:0000785; C:chromatin; ISS:UniProtKB.
 GO:0008278; C:cohesin complex; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0000795; C:synaptonemal complex; IDA:MGI.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0035562; P:negative regulation of chromatin binding; ISS:UniProtKB.
 GO:0008156; P:negative regulation of DNA replication; ISS:UniProtKB.
 GO:0045875; P:negative regulation of sister chromatid cohesion; ISS:UniProtKB.
 GO:0071168; P:protein localization to chromatin; ISS:UniProtKB.
 GO:0071922; P:regulation of cohesin localization to chromatin; ISS:UniProtKB.
 GO:0009636; P:response to toxic substance; IDA:MGI. 
Interpro
 IPR016024; ARM-type_fold.
 IPR012502; WAPL_metazoan_plants.
 IPR022771; WAPL_prot. 
Pfam
 PF07814; WAPL 
SMART
  
PROSITE
 PS51271; WAPL 
PRINTS