UniProt Accession

 NQOR_ECOLI; P0A8G6; P30849; P75890; P77543 

Genbank Protein ID

 M99166; U00096; AP009048 

Genbank Nucleotide ID

 AAA24759.1; AAC74089.1; BAA35771.1 

Protein Name

 NAD(P)H dehydrogenase (quinone) 

Protein Synonyms/Alias

 Flavoprotein WrbA; NAD(P)H:quinone oxidoreductase; NQO 

Gene Name

 wrbA 

Gene Synonyms/Alias

 b1004; JW0989 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
28	AVAEGASKVDGAEVV	acetylation	[1]
37	DGAEVVVKRVPETMP	acetylation	[1, 2]
50	MPPQLFEKAGGKTQT	acetylation	[1, 3]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 

Functional Description

 It seems to function in response to environmental stress when various electron transfer chains are affected or when the environment is highly oxidizing. It reduces quinones to the hydroquinone state to prevent interaction of the semiquinone with O2 and production of superoxide. It prefers NADH over NADPH. 

Sequence Annotation

 DOMAIN 4 189 Flavodoxin-like.
 NP_BIND 9 14 FMN.
 NP_BIND 77 80 FMN.
 NP_BIND 112 118 FMN.
 BINDING 12 12 NAD.
 BINDING 51 51 NAD; via carbonyl oxygen.
 BINDING 98 98 Substrate.
 BINDING 133 133 FMN.  

Keyword

 3D-structure; Complete proteome; Direct protein sequencing; Flavoprotein; FMN; NAD; Nucleotide-binding; Oxidoreductase; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 198 AA 

Protein Sequence

Gene Ontology

 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:HAMAP.
 GO:0010181; F:FMN binding; IDA:EcoCyc.
 GO:0051287; F:NAD binding; IEA:HAMAP.
 GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IDA:EcoCyc.
 GO:0050661; F:NADP binding; IEA:HAMAP.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IEA:InterPro.
 GO:0006979; P:response to oxidative stress; IEP:EcoCyc. 

Interpro

 IPR008254; Flavodoxin/NO_synth.
 IPR010089; Flavoprotein_WrbA. 

Pfam

 PF00258; Flavodoxin_1 

SMART

  

PROSITE

 PS50902; FLAVODOXIN_LIKE 

PRINTS