CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008488
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase polo 
Protein Synonyms/Alias
  
Gene Name
 polo 
Gene Synonyms/Alias
 CG12306 
Created Date
 July 27, 2013 
Organism
 Drosophila melanogaster (Fruit fly) 
NCBI Taxa ID
 7227 
Lysine Modification
Position
Peptide
Type
References
33KRMRFFGKGGFAKCYacetylation[1]
566KIRYAQEKLRKMLEKacetylation[1]
Reference
 [1] Proteome-wide mapping of the Drosophila acetylome demonstrates a high degree of conservation of lysine acetylation.
 Weinert BT, Wagner SA, Horn H, Henriksen P, Liu WR, Olsen JV, Jensen LJ, Choudhary C.
 Sci Signal. 2011 Jul 26;4(183):ra48. [PMID: 21791702] 
Functional Description
 May play a role in regulating both nuclear and cytoplasmic aspects of the mitotic cycle. 
Sequence Annotation
 DOMAIN 25 277 Protein kinase.
 DOMAIN 398 461 POLO box 1.
 DOMAIN 496 564 POLO box 2.
 NP_BIND 31 39 ATP (By similarity).
 ACT_SITE 148 148 Proton acceptor (By similarity).
 BINDING 54 54 ATP (By similarity).
 MOD_RES 182 182 Phosphothreonine.
 MOD_RES 186 186 Phosphothreonine.
 MOD_RES 299 299 Phosphoserine.
 MOD_RES 302 302 Phosphothreonine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 323 323 Phosphothreonine.
 MOD_RES 327 327 Phosphoserine.
 MOD_RES 328 328 Phosphothreonine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 576 AA 
Protein Sequence
MAAKPEDKST DIPDRLVDIN QRKTYKRMRF FGKGGFAKCY EIIDVETDDV FAGKIVSKKL 60
MIKHNQKEKT AQEITIHRSL NHPNIVKFHN YFEDSQNIYI VLELCKKRSM MELHKRRKSI 120
TEFECRYYIY QIIQGVKYLH DNRIIHRDLK LGNLFLNDLL HVKIGDFGLA TRIEYEGERK 180
KTLCGTPNYI APEILTKKGH SFEVDIWSIG CVMYTLLVGQ PPFETKTLKD TYSKIKKCEY 240
RVPSYLRKPA ADMVIAMLQP NPESRPAIGQ LLNFEFLKGS KVPMFLPSSC LTMAPRIGSN 300
DTIEDSMHRK PLMEMNGIRP DDTRLESTFL KANLHDAITA SAQVCRHSED YRSDIESLYQ 360
QLTNLINGKP RILQGNLGDE NTDPAAQPLF WISKWVDYSD KYGFGYQLCD EGIGVMFNDT 420
TKLILLPNQI NVHFIDKDGK ETYMTTTDYC KSLDKKMKLL SYFKRYMIEH LVKAGANNVN 480
IESDQISRMP HLHSWFRTTC AVVMHLTNGS VQLNFSDHMK LILCPRMSAI TYMDQEKNFR 540
TYRFSTIVEN GVSKDLYQKI RYAQEKLRKM LEKMFT 576 
Gene Ontology
 GO:0005814; C:centriole; IDA:FlyBase.
 GO:0000940; C:condensed chromosome outer kinetochore; IDA:FlyBase.
 GO:0005874; C:microtubule; IDA:FlyBase.
 GO:0030496; C:midbody; IDA:FlyBase.
 GO:0005635; C:nuclear envelope; IDA:FlyBase.
 GO:0000922; C:spindle pole; IDA:FlyBase.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:FlyBase.
 GO:0030954; P:astral microtubule nucleation; IMP:FlyBase.
 GO:0051297; P:centrosome organization; IMP:FlyBase.
 GO:0000910; P:cytokinesis; TAS:FlyBase.
 GO:0035050; P:embryonic heart tube development; IMP:FlyBase.
 GO:0007060; P:male meiosis chromosome segregation; TAS:FlyBase.
 GO:0007067; P:mitosis; IMP:FlyBase.
 GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
 GO:0007406; P:negative regulation of neuroblast proliferation; IMP:FlyBase.
 GO:0048600; P:oocyte fate commitment; IMP:FlyBase.
 GO:0006911; P:phagocytosis, engulfment; IMP:FlyBase.
 GO:0007344; P:pronuclear fusion; IMP:FlyBase.
 GO:0035046; P:pronuclear migration; IMP:FlyBase.
 GO:0008104; P:protein localization; NAS:FlyBase.
 GO:0035044; P:sperm aster formation; IMP:FlyBase.
 GO:0007058; P:spindle assembly involved in female meiosis II; IMP:FlyBase. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000959; POLO_box_duplicated_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase
 PF00659; POLO_box 
SMART
 SM00220; S_TKc 
PROSITE
 PS50078; POLO_BOX
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS