CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000704
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 52 kDa repressor of the inhibitor of the protein kinase 
Protein Synonyms/Alias
 p52rIPK; 58 kDa interferon-induced protein kinase-interacting protein; p58IPK-interacting protein; Death-associated protein 4; THAP domain-containing protein 0 
Gene Name
 PRKRIR 
Gene Synonyms/Alias
 DAP4; P52RIPK; THAP0 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
603EQHLKALKCLSLVPSubiquitination[1]
654IKWKHRGKDIELPSTubiquitination[1]
705ENGRKRLKAYLRNTLubiquitination[1]
747YIKLYTSKSELPTDNubiquitination[2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR- inhibitory function of P58IPK, resulting in restoration of kinase activity and suppression of cell growth. 
Sequence Annotation
 ZN_FING 1 86 THAP-type.  
Keyword
 Alternative splicing; Complete proteome; DNA-binding; Metal-binding; Reference proteome; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 761 AA 
Protein Sequence
MPNFCAAPNC TRKSTQSDLA FFRFPRDPAR CQKWVENCRR ADLEDKTPDQ LNKHYRLCAK 60
HFETSMICRT SPYRTVLRDN AIPTIFDLTS HLNNPHSRHR KRIKELSEDE IRTLKQKKID 120
ETSEQEQKHK ETNNSNAQNP SEEEGEGQDE DILPLTLEEK ENKEYLKSLF EILILMGKQN 180
IPLDGHEADE IPEGLFTPDN FQALLECRIN SGEEVLRKRF ETTAVNTLFC SKTQQRQMLE 240
ICESCIREET LREVRDSHFF SIITDDVVDI AGEEHLPVLV RFVDESHNLR EEFIGFLPYE 300
ADAEILAVKF HTMITEKWGL NMEYCRGQAY IVSSGFSSKM KVVASRLLEK YPQAIYTLCS 360
SCALNMWLAK SVPVMGVSVA LGTIEEVCSF FHRSPQLLLE LDNVISVLFQ NSKERGKELK 420
EICHSQWTGR HDAFEILVEL LQALVLCLDG INSDTNIRWN NYIAGRAFVL CSAVSDFDFI 480
VTIVVLKNVL SFTRAFGKNL QGQTSDVFFA AGSLTAVLHS LNEVMENIEV YHEFWFEEAT 540
NLATKLDIQM KLPGKFRRAH QGNLESQLTS ESYYKETLSV PTVEHIIQEL KDIFSEQHLK 600
ALKCLSLVPS VMGQLKFNTS EEHHADMYRS DLPNPDTLSA ELHCWRIKWK HRGKDIELPS 660
TIYEALHLPD IKFFPNVYAL LKVLCILPVM KVENERYENG RKRLKAYLRN TLTDQRSSNL 720
ALLNINFDIK HDLDLMVDTY IKLYTSKSEL PTDNSETVEN T 761 
Gene Ontology
 GO:0005634; C:nucleus; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0006950; P:response to stress; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR025398; DUF4371.
 IPR008906; HATC.
 IPR012337; RNaseH-like_dom.
 IPR006612; Znf_C2CH. 
Pfam
 PF05699; Dimer_Tnp_hAT
 PF14291; DUF4371
 PF05485; THAP 
SMART
 SM00692; DM3
 SM00980; THAP 
PROSITE
 PS50950; ZF_THAP 
PRINTS