CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014315
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Poly(A) polymerase alpha 
Protein Synonyms/Alias
 PAP-alpha; Polynucleotide adenylyltransferase 
Gene Name
 Papola 
Gene Synonyms/Alias
 Pap; Plap 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
444WVIGLVFKKTENSENsumoylation[1]
445VIGLVFKKTENSENLsumoylation[1]
506SHVLQKRKKHSTEGVsumoylation[1]
507HVLQKRKKHSTEGVKsumoylation[1]
730ANPIPVIKNSIKLRLsumoylation[1]
734PVIKNSIKLRLNR**sumoylation[1]
Reference
 [1] Sumoylation regulates multiple aspects of mammalian poly(A) polymerase function.
 Vethantham V, Rao N, Manley JL.
 Genes Dev. 2008 Feb 15;22(4):499-511. [PMID: 18281463
Functional Description
 Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus (By similarity). 
Sequence Annotation
 NP_BIND 100 102 ATP (By similarity).
 NP_BIND 113 115 ATP (By similarity).
 NP_BIND 246 247 ATP (By similarity).
 REGION 508 643 Ser/Thr-rich.
 REGION 671 739 Required for interaction with NUDT21.
 MOTIF 490 507 Nuclear localization signal 1 (By
 MOTIF 644 659 Nuclear localization signal 2 (By
 METAL 113 113 Magnesium 1; catalytic (By similarity).
 METAL 113 113 Magnesium 2; catalytic (By similarity).
 METAL 115 115 Magnesium 1; catalytic (By similarity).
 METAL 115 115 Magnesium 2; catalytic (By similarity).
 METAL 167 167 Magnesium 2; catalytic (By similarity).
 BINDING 109 109 ATP (By similarity).
 BINDING 167 167 ATP (By similarity).
 BINDING 228 228 ATP (By similarity).
 BINDING 237 237 ATP (By similarity).
 MOD_RES 24 24 Phosphoserine (By similarity).
 MOD_RES 537 537 Phosphoserine; by MAPK.
 MOD_RES 635 635 N6-acetyllysine (By similarity).
 MOD_RES 644 644 N6-acetyllysine (By similarity).
 MOD_RES 730 730 N6-acetyllysine; alternate (By
 MOD_RES 734 734 N6-acetyllysine; alternate (By
 CROSSLNK 444 444 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 445 445 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 506 506 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 507 507 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 730 730 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 734 734 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Isopeptide bond; Magnesium; Manganese; Metal-binding; mRNA processing; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 739 AA 
Protein Sequence
MPFPVTTQGS QQTQPPQRHY GITSPISLAA PKETDCLLTQ KLIETLKPFG VFEEEEELQR 60
RILILGKLNN LVKEWIREIS ESKNLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA 120
PRHVDRSDFF TSFYDKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE 180
DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKRHNIYSNI 240
LGFLGGVSWA MLVARTCQLY PNAIASTLVH KFFLVFSKWE WPNPVLLKQP EECNLNLPVW 300
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMVEE FKQGLAITDE ILLSKAEWSK 360
LFEAPNFFQK YKHYIVLLAS APTEKQRLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS 420
FPAPKESPDR EEFRTMWVIG LVFKKTENSE NLSVDLTYDI QSFTDTVYRQ AINSKMFELD 480
MKIAAMHVKR KQLHQLLPSH VLQKRKKHST EGVKLTALND SSLDLSMDSD NSMSVPSPTS 540
AMKTSPLNSS GSSQGRNSPA PAVTAASVTS IQASEVSVPQ ANSSESPGGP SSESIPQTAT 600
QPAISPPPKP TVSRVVSSTR LVNPSPRPSG NTATKVPNPI VGVKRTSSPN KEESPKKTKT 660
EEDETSEDAN CLALSGHDKT ETKEQVDLET SAVQSETVPA SASLLASQKT SSTDLSDIPA 720
LPANPIPVIK NSIKLRLNR 739 
Gene Ontology
 GO:0005737; C:cytoplasm; IBA:RefGenome.
 GO:0005634; C:nucleus; IBA:RefGenome.
 GO:0005524; F:ATP binding; ISS:UniProtKB.
 GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
 GO:0030145; F:manganese ion binding; ISS:UniProtKB.
 GO:0004652; F:polynucleotide adenylyltransferase activity; ISS:UniProtKB.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006378; P:mRNA polyadenylation; IBA:RefGenome.
 GO:0000398; P:mRNA splicing, via spliceosome; IBA:RefGenome.
 GO:0006369; P:termination of RNA polymerase II transcription; IBA:RefGenome. 
Interpro
 IPR002934; Nucleotidyltransferase.
 IPR011068; NuclTrfase_I_C.
 IPR007012; PolA_pol_cen_dom.
 IPR007010; PolA_pol_RNA-bd_dom.
 IPR014492; PolyA_polymerase. 
Pfam
 PF01909; NTP_transf_2
 PF04928; PAP_central
 PF04926; PAP_RNA-bind 
SMART
  
PROSITE
  
PRINTS