UniProt Accession

 NANA_ECOLI; P0A6L4; P06995; Q2M8Y8 

Genbank Protein ID

 X03345; D00067; U18997; U00096; AP009048 

Genbank Nucleotide ID

 CAA27051.1; BAA00046.1; AAA58027.1; AAC76257.1; BAE77268.1 

Protein Name

 N-acetylneuraminate lyase 

Protein Synonyms/Alias

 N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; NALase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase 

Gene Name

 nanA 

Gene Synonyms/Alias

 npl; b3225; JW3194 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
24	DQQQALDKASLRRLV	acetylation	[1]
165	LPGVGALKQTSGDLY	acetylation	[2]
280	PFGPVDEKYLPELKA	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. 

Sequence Annotation

 REGION 47 48 Substrate binding.
 ACT_SITE 165 165 Schiff-base intermediate with substrate.  

Keyword

 3D-structure; Carbohydrate metabolism; Complete proteome; Cytoplasm; Direct protein sequencing; Lyase; Reference proteome; Schiff base. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 297 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0008747; F:N-acetylneuraminate lyase activity; IDA:EcoCyc.
 GO:0005975; P:carbohydrate metabolic process; IEA:HAMAP.
 GO:0019262; P:N-acetylneuraminate catabolic process; IMP:EcoCyc. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR002220; Dihydrodipicolinate_synth-like.
 IPR020625; Dihydrodipicolinate_synth_AS.
 IPR020624; Dihydrodipicolinate_synth_CS.
 IPR005264; NanA. 

Pfam

 PF00701; DHDPS 

SMART

  

PROSITE

 PS00665; DHDPS_1
 PS00666; DHDPS_2 

PRINTS

 PR00146; DHPICSNTHASE.