CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001334
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exostosin-2 
Protein Synonyms/Alias
 Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase; HS-polymerase; HS-POL; Multiple exostoses protein 2 homolog 
Gene Name
 EXT2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Bos taurus (Bovine) 
NCBI Taxa ID
 9913 
Lysine Modification
Position
Peptide
Type
References
478RVITEVSKVPSLSKLubiquitination[1]
504PEDSLWPKIRVPLKVubiquitination[1]
510PKIRVPLKVVRTAENubiquitination[1]
518VVRTAENKLSNRFFPubiquitination[1]
579LWDHEMNKWKYESEWubiquitination[1]
581DHEMNKWKYESEWTNubiquitination[1]
645VTGKAVIKVTPRKKFubiquitination[1]
653VTPRKKFKCPECTAIubiquitination[1]
679ERSECINKFASVFGTubiquitination[1, 2]
690VFGTMPLKVVEHRADubiquitination[1, 3]
708YKDDFPEKLKSFPNIubiquitination[1]
710DDFPEKLKSFPNIGSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N- acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains. 
Sequence Annotation
 CARBOHYD 288 288 N-linked (GlcNAc...) (Potential).
 CARBOHYD 637 637 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Golgi apparatus; Membrane; Reference proteome; Secreted; Signal-anchor; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 718 AA 
Protein Sequence
MCASVKYNIR GPALIPRMKT KHRIYYITLF SIVLLGLIAT GMFQFWPHSI ESSGDWSVEK 60
RTGRDVPLVR LPADSPVPER GDLSCRMHTC FDVYRCGFNP KNKIKVYIYP LKKYVGEAGV 120
PVSSTISREY NELLTAISDS DYYTDDVTRA CLFVPSIDLL NQNSLRVKET AQALAQLSRW 180
DRGTNHLLFN MLPGGPPDYN TALDVPRDRA LLAGGGFSTW TYRQGYDVSI PVYSPLSAEV 240
DLPEKGPGPR RYFLLSSQVA LHPEYREDLA ALQARHGEAV LVLDKCSNLS EGVPAARRRC 300
HQQQAFDYPQ VLQEATFCMV LRGARLGQAV LSDVLRAGCV PVIIADSYVL PFSEVLDWKR 360
ASVVVPEEKM SDVYSILQSI PRRQIEEMQR QARWFWEAYF QSIKAIALAT LQIINDRIYP 420
YAAISYEDWN DPPAVKWGSV SNPLFLPLIP PQSQGFTAIV LTYDRVESLF RVITEVSKVP 480
SLSKLLVVWN NQNKNPPEDS LWPKIRVPLK VVRTAENKLS NRFFPYDEIE TEAVLAIDDD 540
IIMLTSDELQ FGYEVWREFP DRLVGYPGRL HLWDHEMNKW KYESEWTNEV SMVLTGAAFY 600
HKYFNYLYTY KMPGDIKNWV DAHMNCEDIA MNFLVANVTG KAVIKVTPRK KFKCPECTAI 660
DGLSLDQTHM VERSECINKF ASVFGTMPLK VVEHRADPVL YKDDFPEKLK SFPNIGSL 718 
Gene Ontology
 GO:0005615; C:extracellular space; IDA:BHF-UCL.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; NAS:UniProtKB.
 GO:0031227; C:intrinsic to endoplasmic reticulum membrane; IEA:InterPro.
 GO:0050508; F:glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity; IDA:UniProtKB.
 GO:0050509; F:N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity; IDA:UniProtKB.
 GO:0006044; P:N-acetylglucosamine metabolic process; IDA:UniProtKB.
 GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway. 
Interpro
 IPR004263; Exostosin.
 IPR015338; HexNAc_Trfase_a. 
Pfam
 PF03016; Exostosin
 PF09258; Glyco_transf_64 
SMART
  
PROSITE
  
PRINTS