CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012081
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Unconventional myosin-VIIa 
Protein Synonyms/Alias
  
Gene Name
 MYO7A 
Gene Synonyms/Alias
 USH1B 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
693RVLLPGVKPAYKQGDubiquitination[1]
1256LQATKSKKPIMLPVTubiquitination[2]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. In the retina, plays an important role in the renewal of the outer photoreceptor disks. Plays an important role in the distribution and migration of retinal pigment epithelial (RPE) melanosomes and phagosomes, and in the regulation of opsin transport in retinal photoreceptors. In the inner ear, plays an important role in differentiation, morphogenesis and organization of cochlear hair cell bundles. Involved in hair-cell vesicle trafficking of aminoglycosides, which are known to induce ototoxicity (By similarity). Motor protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. 
Sequence Annotation
 DOMAIN 1 729 Myosin head-like.
 DOMAIN 745 765 IQ 1.
 DOMAIN 768 788 IQ 2.
 DOMAIN 791 811 IQ 3.
 DOMAIN 814 834 IQ 4.
 DOMAIN 837 857 IQ 5.
 DOMAIN 1017 1253 MyTH4 1.
 DOMAIN 1258 1602 FERM 1.
 DOMAIN 1603 1672 SH3.
 DOMAIN 1747 1896 MyTH4 2.
 DOMAIN 1902 2205 FERM 2.
 NP_BIND 158 165 ATP (Probable).
 REGION 632 639 Actin-binding (Probable).
 MOD_RES 965 965 Phosphoserine (By similarity).  
Keyword
 Actin-binding; Alternative splicing; ATP-binding; Calmodulin-binding; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Deafness; Disease mutation; Hearing; Leber congenital amaurosis; Motor protein; Myosin; Non-syndromic deafness; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Retinitis pigmentosa; SH3 domain; Usher syndrome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2215 AA 
Protein Sequence
MVILQQGDHV WMDLRLGQEF DVPIGAVVKL CDSGQVQVVD DEDNEHWISP QNATHIKPMH 60
PTSVHGVEDM IRLGDLNEAG ILRNLLIRYR DHLIYTYTGS ILVAVNPYQL LSIYSPEHIR 120
QYTNKKIGEM PPHIFAIADN CYFNMKRNSR DQCCIISGES GAGKTESTKL ILQFLAAISG 180
QHSWIEQQVL EATPILEAFG NAKTIRNDNS SRFGKYIDIH FNKRGAIEGA KIEQYLLEKS 240
RVCRQALDER NYHVFYCMLE GMSEDQKKKL GLGQASDYNY LAMGNCITCE GRVDSQEYAN 300
IRSAMKVLMF TDTENWEISK LLAAILHLGN LQYEARTFEN LDACEVLFSP SLATAASLLE 360
VNPPDLMSCL TSRTLITRGE TVSTPLSREQ ALDVRDAFVK GIYGRLFVWI VDKINAAIYK 420
PPSQDVKNSR RSIGLLDIFG FENFAVNSFE QLCINFANEH LQQFFVRHVF KLEQEEYDLE 480
SIDWLHIEFT DNQDALDMIA NKPMNIISLI DEESKFPKGT DTTMLHKLNS QHKLNANYIP 540
PKNNHETQFG INHFAGIVYY ETQGFLEKNR DTLHGDIIQL VHSSRNKFIK QIFQADVAMG 600
AETRKRSPTL SSQFKRSLEL LMRTLGACQP FFVRCIKPNE FKKPMLFDRH LCVRQLRYSG 660
MMETIRIRRA GYPIRYSFVE FVERYRVLLP GVKPAYKQGD LRGTCQRMAE AVLGTHDDWQ 720
IGKTKIFLKD HHDMLLEVER DKAITDRVIL LQKVIRGFKD RSNFLKLKNA ATLIQRHWRG 780
HNCRKNYGLM RLGFLRLQAL HRSRKLHQQY RLARQRIIQF QARCRAYLVR KAFRHRLWAV 840
LTVQAYARGM IARRLHQRLR AEYLWRLEAE KMRLAEEEKL RKEMSAKKAK EEAERKHQER 900
LAQLAREDAE RELKEKEAAR RKKELLEQME RARHEPVNHS DMVDKMFGFL GTSGGLPGQE 960
GQAPSGFEDL ERGRREMVEE DLDAALPLPD EDEEDLSEYK FAKFAATYFQ GTTTHSYTRR 1020
PLKQPLLYHD DEGDQLAALA VWITILRFMG DLPEPKYHTA MSDGSEKIPV MTKIYETLGK 1080
KTYKRELQAL QGEGEAQLPE GQKKSSVRHK LVHLTLKKKS KLTEEVTKRL HDGESTVQGN 1140
SMLEDRPTSN LEKLHFIIGN GILRPALRDE IYCQISKQLT HNPSKSSYAR GWILVSLCVG 1200
CFAPSEKFVK YLRNFIHGGP PGYAPYCEER LRRTFVNGTR TQPPSWLELQ ATKSKKPIML 1260
PVTFMDGTTK TLLTDSATTA KELCNALADK ISLKDRFGFS LYIALFDKVS SLGSGSDHVM 1320
DAISQCEQYA KEQGAQERNA PWRLFFRKEV FTPWHSPSED NVATNLIYQQ VVRGVKFGEY 1380
RCEKEDDLAE LASQQYFVDY GSEMILERLL NLVPTYIPDR EITPLKTLEK WAQLAIAAHK 1440
KGIYAQRRTD AQKVKEDVVS YARFKWPLLF SRFYEAYKFS GPSLPKNDVI VAVNWTGVYF 1500
VDEQEQVLLE LSFPEIMAVS SSRECRVWLS LGCSDLGCAA PHSGWAGLTP AGPCSPCWSC 1560
RGAKTTAPSF TLATIKGDEY TFTSSNAEDI RDLVVTFLEG LRKRSKYVVA LQDNPNPAGE 1620
ESGFLSFAKG DLIILDHDTG EQVMNSGWAN GINERTKQRG DFPTDSVYVM PTVTMPPREI 1680
VALVTMTPDQ RQDVVRLLQL RTAEPEVRAK PYTLEEFSYD YFRPPPKHTL SRVMVSKARG 1740
KDRLWSHTRE PLKQALLKKL LGSEELSQEA CLAFIAVLKY MGDYPSKRTR SVNELTDQIF 1800
EGPLKAEPLK DEAYVQILKQ LTDNHIRYSE ERGWELLWLC TGLFPPSNIL LPHVQRFLQS 1860
RKHCPLAIDC LQRLQKALRN GSRKYPPHLV EVEAIQHKTT QIFHKVYFPD DTDEAFEVES 1920
STKAKDFCQN IATRLLLKSS EGFSLFVKIA DKVLSVPEND FFFDFVRHLT DWIKKARPIK 1980
DGIVPSLTYQ VFFMKKLWTT TVPGKDPMAD SIFHYYQELP KYLRGYHKCT REEVLQLGAL 2040
IYRVKFEEDK SYFPSIPKLL RELVPQDLIR QVSPDDWKRS IVAYFNKHAG KSKEEAKLAF 2100
LKLIFKWPTF GSAFFEVKQT TEPNFPEILL IAINKYGVSL IDPKTKDILT THPFTKISNW 2160
SSGNTYFHIT IGNLVRGSKL LCETSLGYKM DDLLTSYISQ MLTAMSKQRG SRSGK 2215 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0005938; C:cell cortex; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
 GO:0042470; C:melanosome; IEA:Compara.
 GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
 GO:0032391; C:photoreceptor connecting cilium; IEA:Compara.
 GO:0001917; C:photoreceptor inner segment; IDA:UniProtKB.
 GO:0001750; C:photoreceptor outer segment; IDA:UniProtKB.
 GO:0032420; C:stereocilium; IEA:Compara.
 GO:0045202; C:synapse; IDA:UniProtKB.
 GO:0051015; F:actin filament binding; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005516; F:calmodulin binding; IMP:UniProtKB.
 GO:0000146; F:microfilament motor activity; IDA:UniProtKB.
 GO:0030048; P:actin filament-based movement; IDA:UniProtKB.
 GO:0060088; P:auditory receptor cell stereocilium organization; IEA:Compara.
 GO:0050957; P:equilibrioception; IMP:HGNC.
 GO:0042462; P:eye photoreceptor cell development; IC:UniProtKB.
 GO:0006886; P:intracellular protein transport; IEA:Compara.
 GO:0007040; P:lysosome organization; IDA:UniProtKB.
 GO:0001845; P:phagolysosome assembly; IEA:Compara.
 GO:0051904; P:pigment granule transport; IEA:Compara.
 GO:0048563; P:post-embryonic organ morphogenesis; IEA:Compara.
 GO:0007605; P:sensory perception of sound; IMP:UniProtKB.
 GO:0007601; P:visual perception; IMP:UniProtKB. 
Interpro
 IPR019749; Band_41_domain.
 IPR014352; FERM/acyl-CoA-bd_prot_3-hlx.
 IPR019748; FERM_central.
 IPR000299; FERM_domain.
 IPR018979; FERM_N.
 IPR000048; IQ_motif_EF-hand-BS.
 IPR001609; Myosin_head_motor_dom.
 IPR000857; MyTH4_dom.
 IPR027417; P-loop_NTPase.
 IPR011993; PH_like_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00373; FERM_M
 PF09379; FERM_N
 PF00612; IQ
 PF00063; Myosin_head
 PF00784; MyTH4 
SMART
 SM00295; B41
 SM00015; IQ
 SM00242; MYSc
 SM00139; MyTH4
 SM00326; SH3 
PROSITE
 PS00660; FERM_1
 PS00661; FERM_2
 PS50057; FERM_3
 PS50096; IQ
 PS51016; MYTH4
 PS50002; SH3 
PRINTS
 PR00193; MYOSINHEAVY.