UniProt Accession

 AIF1_MOUSE; O70200 

Genbank Protein ID

 AB013745; AB036423; D86382; AF074959; U82792; AK006184; AK006562; AF109719; BC021539 

Genbank Nucleotide ID

 BAA28216.1; BAB20758.1; BAA86387.1; AAC25604.1; AAC24189.1; BAB24445.1; BAB24654.1; AAC82481.1; AAH21539.1 

Protein Name

 Allograft inflammatory factor 1 

Protein Synonyms/Alias

 AIF-1; Ionized calcium-binding adapter molecule 1 

Gene Name

 Aif1 

Gene Synonyms/Alias

 Iba1 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
76	SLKRMLEKLGVPKTH	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 

Sequence Annotation

 DOMAIN 45 80 EF-hand 1.
 DOMAIN 81 115 EF-hand 2; degenerate.
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 11 11 N6-acetyllysine (By similarity).  

Keyword

 3D-structure; Acetylation; Actin-binding; Calcium; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 147 AA 

Protein Sequence

Gene Ontology

 GO:0005884; C:actin filament; IDA:MGI.
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0001891; C:phagocytic cup; IDA:UniProtKB.
 GO:0032587; C:ruffle membrane; IDA:UniProtKB.
 GO:0051015; F:actin filament binding; ISS:UniProtKB.
 GO:0005509; F:calcium ion binding; IDA:UniProtKB.
 GO:0051017; P:actin filament bundle assembly; ISS:UniProtKB.
 GO:0030041; P:actin filament polymerization; ISS:UniProtKB.
 GO:0071346; P:cellular response to interferon-gamma; ISS:UniProtKB.
 GO:0006954; P:inflammatory response; ISS:UniProtKB.
 GO:0001774; P:microglial cell activation; NAS:UniProtKB.
 GO:0071672; P:negative regulation of smooth muscle cell chemotaxis; IEA:Compara.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0006911; P:phagocytosis, engulfment; IMP:UniProtKB.
 GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; ISS:UniProtKB.
 GO:0090026; P:positive regulation of monocyte chemotaxis; ISS:UniProtKB.
 GO:0071673; P:positive regulation of smooth muscle cell chemotaxis; ISS:UniProtKB.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
 GO:2000406; P:positive regulation of T cell migration; ISS:UniProtKB.
 GO:0042102; P:positive regulation of T cell proliferation; ISS:UniProtKB.
 GO:0016601; P:Rac protein signal transduction; IMP:UniProtKB.
 GO:0097178; P:ruffle assembly; IMP:UniProtKB. 

Interpro

 IPR011992; EF-hand-like_dom.
 IPR002048; EF_hand_dom. 

Pfam

  

SMART

  

PROSITE

 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 

PRINTS