CPLM-021759

Genbank Nucleotide ID

Na(+)/H(+) exchange regulatory cofactor NHE-RF3

Protein Synonyms/Alias

NHERF-3; CFTR-associated protein of 70 kDa; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; PDZ domain-containing protein 1; Sodium-hydrogen exchanger regulatory factor 3

Mus musculus (Mouse)

Position	Peptide	Type	References
14	PRECKLSKQEGQNYG	ubiquitination	[1]
28	GFFLRIEKDTDGHLI	ubiquitination	[1]
46	EEGSPAEKAGLLDGD	ubiquitination	[1]
65	INGVFVDKEEHAQVV	ubiquitination	[1]
77	QVVELVRKSGNSVTL	ubiquitination	[1]
94	LDGDSYEKAVKNQVD	ubiquitination	[1]
97	DSYEKAVKNQVDLKE	ubiquitination	[1]
103	VKNQVDLKELDQSQR	ubiquitination	[1]
117	REAALNDKKPGPGMN	ubiquitination	[1]
118	EAALNDKKPGPGMNG	ubiquitination	[1]
150	NSFGFSLKTIQGKKG	ubiquitination	[1]
156	LKTIQGKKGVYLTDI	ubiquitination	[1]
171	MPQGVAMKAGVLADD	ubiquitination	[1]
199	SHEEVVEKVTKSGSR	ubiquitination	[1]
214	IMFLLVDKETARCHS	ubiquitination	[1]
224	ARCHSEQKTQFKRET	ubiquitination	[1]
235	KRETASLKLLPHQPR	ubiquitination	[1]
248	PRVVVIKKGSNGYGF	ubiquitination	[1]
264	LRAGPEQKGQIIKDI	ubiquitination	[1]
269	EQKGQIIKDIEPGSP	ubiquitination	[1]
283	PAEAAGLKNNDLVVA	ubiquitination	[1]
294	LVVAVNGKSVEALDH	ubiquitination	[1]
310	GVVEMIRKGGDQTTL	ubiquitination	[1]
322	TTLLVLDKEAESIYS	ubiquitination	[1]
350	ELPNGSVKEGPAPIP	ubiquitination	[1]
375	TEDAEGHKPKLCRLL	ubiquitination	[1]
383	PKLCRLLKEDDSYGF	ubiquitination	[1]
404	GQPGSFVKEVQQGGP	ubiquitination	[1]
414	QQGGPADKAGLENED	ubiquitination	[1]
448	ERIKSSGKHVTLLVC	ubiquitination	[1]
466	AYSYFQAKKIPIVSS	ubiquitination	[1]
467	YSYFQAKKIPIVSSM	ubiquitination	[1]
485	LVAGPDEKGETSAES	ubiquitination	[1]
500	EHDAHPAKDRTLSTA	ubiquitination	[1]

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism (By similarity). May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.

DOMAIN 9 90 PDZ 1.
DOMAIN 128 215 PDZ 2.
DOMAIN 243 323 PDZ 3.
DOMAIN 378 458 PDZ 4.
MOD_RES 488 488 Phosphothreonine.
MOD_RES 489 489 Phosphoserine.
MOD_RES 492 492 Phosphoserine.
MOD_RES 514 514 Phosphoserine.

3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Phosphoprotein; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031526; C:brush border membrane; IDA:BHF-UCL.
GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
GO:0015879; P:carnitine transport; IEA:Compara.
GO:0090002; P:establishment of protein localization to plasma membrane; IMP:UniProtKB.
GO:0034767; P:positive regulation of ion transmembrane transport; IEA:Compara.