CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-020035
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamate-rich WD repeat-containing protein 1 
Protein Synonyms/Alias
  
Gene Name
 GRWD1 
Gene Synonyms/Alias
 GRWD; KIAA1942; WDR28 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
172VAGVWSEKGQVEVFAubiquitination[1]
237LLTGDCQKNIHLWTPubiquitination[2]
297DIRAAPSKACMLTTAubiquitination[2]
333GGDDGALKIWDLRQFubiquitination[2, 3, 4]
341IWDLRQFKSGSPVATubiquitination[2, 5]
Reference
 [1] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
 REPEAT 212 252 WD 1.
 REPEAT 259 299 WD 2.
 REPEAT 306 345 WD 3.
 REPEAT 351 391 WD 4.
 REPEAT 412 445 WD 5.
 MOD_RES 10 10 Phosphothreonine.
 MOD_RES 119 119 Phosphoserine.
 MOD_RES 122 122 Phosphoserine.  
Keyword
 Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 446 AA 
Protein Sequence
MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD EEAYVLYHRA 60
QTGAPCLSFD IVRDHLGDNR TELPLTLYLC AGTQAESAQS NRLMMLRMHN LHGTKPPPSE 120
GSDEEEEEED EEDEEERKPQ LELAMVPHYG GINRVRVSWL GEEPVAGVWS EKGQVEVFAL 180
RRLLQVVEEP QALAAFLRDE QAQMKPIFSF AGHMGEGFAL DWSPRVTGRL LTGDCQKNIH 240
LWTPTDGGSW HVDQRPFVGH TRSVEDLQWS PTENTVFASC SADASIRIWD IRAAPSKACM 300
LTTATAHDGD VNVISWSRRE PFLLSGGDDG ALKIWDLRQF KSGSPVATFK QHVAPVTSVE 360
WHPQDSGVFA ASGADHQITQ WDLAVERDPE AGDVEADPGL ADLPQQLLFV HQGETELKEL 420
HWHPQCPGLL VSTALSGFTI FRTISV 446 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR022052; Histone-bd_RBBP4.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF12265; CAF1C_H4-bd
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.