UniProt Accession

 OSBP1_HUMAN; P22059; Q6P524 

Genbank Protein ID

 M86917; AF185696; AF185705; AF185697; AF185698; AF185699; AF185700; AF185701; AF185702; AF185703; AF185704; BC011581; BC063121 

Genbank Nucleotide ID

 AAA59973.1; AAG17011.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAG28373.1; AAH11581.1; AAH63121.1 

Protein Name

 Oxysterol-binding protein 1 

Protein Synonyms/Alias

  

Gene Name

 OSBP 

Gene Synonyms/Alias

 OSBP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
103	FKWTNYIKGYQRRWF	acetylation	[1]
230	TCNDLIAKHGTALQR	ubiquitination	[2]
292	THSKKWQKSLQYERD	ubiquitination	[3]
429	IMKNCIGKELSKIPM	ubiquitination	[4]
433	CIGKELSKIPMPVNF	ubiquitination	[2, 5]
493	TTVFRTSKPFNPLLG	ubiquitination	[5]
544	WTLRQEIKITSKFRG	ubiquitination	[2]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25- hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. 

Sequence Annotation

 DOMAIN 88 181 PH.
 REGION 406 457 Sterol binding (By similarity).
 MOD_RES 103 103 N6-acetyllysine.
 MOD_RES 190 190 Phosphoserine.
 MOD_RES 193 193 Phosphoserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 238 238 Phosphoserine (By similarity).
 MOD_RES 351 351 Phosphoserine.
 MOD_RES 377 377 Phosphothreonine (By similarity).
 MOD_RES 379 379 Phosphoserine (By similarity).
 MOD_RES 382 382 Phosphoserine.
 MOD_RES 389 389 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Coiled coil; Complete proteome; Cytoplasm; Golgi apparatus; Lipid transport; Lipid-binding; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 807 AA 

Protein Sequence

Gene Ontology

 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0008142; F:oxysterol binding; TAS:ProtInc.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0006869; P:lipid transport; IEA:UniProtKB-KW. 

Interpro

 IPR000648; Oxysterol-bd.
 IPR018494; Oxysterol-bd_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology. 

Pfam

 PF01237; Oxysterol_BP
 PF00169; PH 

SMART

 SM00233; PH 

PROSITE

 PS01013; OSBP
 PS50003; PH_DOMAIN 

PRINTS