CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023825
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein FAM115A 
Protein Synonyms/Alias
  
Gene Name
 FAM115A 
Gene Synonyms/Alias
 KIAA0738 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
113EGSGVDAKVEPEVKDubiquitination[1, 2]
119AKVEPEVKDSLGVYCubiquitination[2]
143EKLVKFMKCGGGLLIubiquitination[2]
194KGDTSFFKVSKKMPKubiquitination[1]
274RVVVTGHKVLFTVGKubiquitination[1, 2, 3, 4]
281KVLFTVGKLGPFLLNubiquitination[2, 5, 6]
406PGPFRTPKAGIRTYHubiquitination[1]
434RKRGNVEKGWLAKLGubiquitination[1, 2, 3, 4, 6, 7]
439VEKGWLAKLGPDGAAubiquitination[1, 6, 7]
632GSVPVTVKGAVHAPYubiquitination[2, 7]
641AVHAPYYKLGETTLEubiquitination[2]
651ETTLEEWKRRIQENPubiquitination[1, 2, 6, 8, 9]
750NEKLIRTKGLWGPVHubiquitination[1, 2, 3, 4, 5, 6, 7, 9]
809LWPPVREKRVRIYLSubiquitination[6]
817RVRIYLSKGPNVKNWubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 10, 11]
865LPTENVDKMNLWVKMubiquitination[1, 3, 4, 6]
907LAYLPEWKENIMKLYubiquitination[1, 6]
912EWKENIMKLYLLTQMubiquitination[1, 2, 3, 4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [11] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572
Functional Description
  
Sequence Annotation
  
Keyword
 Alternative splicing; Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 921 AA 
Protein Sequence
MATPSAAFEA LMNGVTSWDV PEDAVPCELL LIGEASFPVM VNDMGQVLIA ASSYGRGRLV 60
VVSHEDYLVE AQLTPFLLNA VGWLCSSPGA PIGVHPSLAP LAKILEGSGV DAKVEPEVKD 120
SLGVYCIDAY NETMTEKLVK FMKCGGGLLI GGQAWDWANQ GEDERVLFTF PGNLVTSVAG 180
IYFTDNKGDT SFFKVSKKMP KIPVLVSCED DLSDDREELL HGISELDISN SDCFPSQLLV 240
HGALAFPLGL DSYHGCVIAA ARYGRGRVVV TGHKVLFTVG KLGPFLLNAV RWLDGGRRGK 300
VVVQTELRTL SGLLAVGGID TSIEPNLTSD ASVYCFEPVS EVGVKELQEF VAEGGGLFVG 360
AQAWWWAFKN PGVSPLARFP GNLLLNPFGI SITSQSLNPG PFRTPKAGIR TYHFRSTLAE 420
FQVIMGRKRG NVEKGWLAKL GPDGAAFLQI PAEEIPAYMS VHRLLRKLLS RYRLPVATRE 480
NPVINDCCRG AMLSLATGLA HSGSDLSLLV PEIEDMYSSP YLRPSESPIT VEVNCTNPGT 540
RYCWMSTGLY IPGRQIIEVS LPEAAASADL KIQIGCHTDD LTRASKLFRG PLVINRCCLD 600
KPTKSITCLW GGLLYIIVPQ NSKLGSVPVT VKGAVHAPYY KLGETTLEEW KRRIQENPGP 660
WGELATDNII LTVPTANLRT LENPEPLLRL WDEVMQAVAR LGAEPFPLRL PQRIVADVQI 720
SVGWMHAGYP IMCHLESVQE LINEKLIRTK GLWGPVHELG RNQQRQEWEF PPHTTEATCN 780
LWCVYVHETV LGIPRSRANI ALWPPVREKR VRIYLSKGPN VKNWNAWTAL ETYLQLQEAF 840
GWEPFIRLFT EYRNQTNLPT ENVDKMNLWV KMFSHQVQKN LAPFFEAWAW PIQKEVATSL 900
AYLPEWKENI MKLYLLTQMP H 921 
Gene Ontology
  
Interpro
  
Pfam
  
SMART
  
PROSITE
  
PRINTS