CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002729
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Argininosuccinate synthase 
Protein Synonyms/Alias
 Citrulline--aspartate ligase 
Gene Name
 Ass1 
Gene Synonyms/Alias
 Ass 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
41YLANIGQKEDFEEARacetylation[1]
53EARKKALKLGAKKVFacetylation[1]
58ALKLGAKKVFIEDVSacetylation[1]
112IAQREGAKYVSHGATacetylation[1]
155PEFYNRFKGRNDLMEacetylation[1]
165NDLMEYAKQHGIPIPacetylation[1]
209APPGLYTKTQDPAKAacetylation[1]
215TKTQDPAKAPNTPDVacetylation[1]
228DVLEIEFKKGVPVKVacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Is indirectly involved in the control of blood pressure. 
Sequence Annotation
 NP_BIND 10 18 ATP (By similarity).
 NP_BIND 115 123 ATP (By similarity).
 BINDING 36 36 ATP; via amide nitrogen and carbonyl
 BINDING 87 87 Citrulline (By similarity).
 BINDING 92 92 Citrulline (By similarity).
 BINDING 119 119 Aspartate (By similarity).
 BINDING 123 123 Aspartate (By similarity).
 BINDING 123 123 Citrulline (By similarity).
 BINDING 124 124 Aspartate (By similarity).
 BINDING 127 127 Citrulline (By similarity).
 BINDING 180 180 Citrulline (By similarity).
 BINDING 189 189 Citrulline (By similarity).
 BINDING 270 270 Citrulline (By similarity).
 BINDING 282 282 Citrulline (By similarity).
 MOD_RES 180 180 Phosphoserine (By similarity).
 MOD_RES 219 219 Phosphothreonine (By similarity).  
Keyword
 Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Phosphoprotein; Reference proteome; Urea cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 412 AA 
Protein Sequence
MSSKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF 60
IEDVSKEFVE EFIWPAVQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG 120
KGNDQVRFEL TCYSLAPQIK VIAPWRMPEF YNRFKGRNDL MEYAKQHGIP IPVTPKSPWS 180
MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNTP DVLEIEFKKG VPVKVTNVKD 240
GTTHSTSLDL FMYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 300
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIDK SQERVEGKVQ VSVFKGQVYI 360
LGRESPLSLY NEELVSMNVQ GDYEPIDATG FININSLRLK EYHRLQSKVT AK 412 
Gene Ontology
 GO:0070852; C:cell body fiber; IDA:RGD.
 GO:0005783; C:endoplasmic reticulum; IDA:RGD.
 GO:0005764; C:lysosome; IDA:RGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:RGD.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0043204; C:perikaryon; IDA:RGD.
 GO:0004055; F:argininosuccinate synthase activity; IDA:RGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0015643; F:toxic substance binding; IPI:RGD.
 GO:0006953; P:acute-phase response; IEP:RGD.
 GO:0007568; P:aging; IEP:RGD.
 GO:0006526; P:arginine biosynthetic process; IDA:RGD.
 GO:0000053; P:argininosuccinate metabolic process; IDA:RGD.
 GO:0071418; P:cellular response to amine stimulus; IEP:RGD.
 GO:0071230; P:cellular response to amino acid stimulus; IEP:RGD.
 GO:0071242; P:cellular response to ammonium ion; IEP:RGD.
 GO:0071320; P:cellular response to cAMP; IEP:RGD.
 GO:0071549; P:cellular response to dexamethasone stimulus; IEP:RGD.
 GO:0071377; P:cellular response to glucagon stimulus; IEP:RGD.
 GO:0071346; P:cellular response to interferon-gamma; IEP:RGD.
 GO:0071222; P:cellular response to lipopolysaccharide; IEP:RGD.
 GO:0071400; P:cellular response to oleic acid; IEP:RGD.
 GO:0071356; P:cellular response to tumor necrosis factor; IEP:RGD.
 GO:0060539; P:diaphragm development; IEP:RGD.
 GO:0001822; P:kidney development; IEP:RGD.
 GO:0001889; P:liver development; IEP:RGD.
 GO:0007494; P:midgut development; IEP:RGD.
 GO:0042493; P:response to drug; IEP:RGD.
 GO:0032355; P:response to estradiol stimulus; IEP:RGD.
 GO:0060416; P:response to growth hormone stimulus; IEP:RGD.
 GO:0010046; P:response to mycotoxin; IEP:RGD.
 GO:0007584; P:response to nutrient; IEP:RGD.
 GO:0010043; P:response to zinc ion; IDA:RGD.
 GO:0000050; P:urea cycle; IDA:RGD. 
Interpro
 IPR001518; Arginosuc_synth.
 IPR018223; Arginosuc_synth_CS.
 IPR023434; Arginosuc_synth_type_1_subfam.
 IPR024074; AS_cat/multimer_dom_body.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00764; Arginosuc_synth 
SMART
  
PROSITE
 PS00564; ARGININOSUCCIN_SYN_1
 PS00565; ARGININOSUCCIN_SYN_2 
PRINTS