UniProt Accession

 EAA2_RAT; P31596 

Genbank Protein ID

 X67857; U15098; U15098; AF297648 

Genbank Nucleotide ID

 CAA48042.1; AAA93061.1; AAA93062.1; AAG13411.1 

Protein Name

 Excitatory amino acid transporter 2 

Protein Synonyms/Alias

 GLT-1; Sodium-dependent glutamate/aspartate transporter 2; GLUT-R; Solute carrier family 1 member 2 

Gene Name

 Slc1a2 

Gene Synonyms/Alias

 Eaat2; Glt1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
12	EGANNMPKQVEVRMH	ubiquitination	[1]
29	HLSSEEPKHRNLGMR	acetylation	[2]
29	HLSSEEPKHRNLGMR	ubiquitination	[1]
517	HEDIEMTKTQSVYDD	ubiquitination	[1]
526	QSVYDDTKNHRESNS	ubiquitination	[1]
570	SVEEEPWKREK****	sumoylation	[3]

Reference

 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]
 [2] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [3] A caspase-3-cleaved fragment of the glial glutamate transporter EAAT2 is sumoylated and targeted to promyelocytic leukemia nuclear bodies in mutant SOD1-linked amyotrophic lateral sclerosis.
 Gibb SL, Boston-Howes W, Lavina ZS, Gustincich S, Brown RH Jr, Pasinelli P, Trotti D.
 J Biol Chem. 2007 Nov 2;282(44):32480-90. [PMID: 17823119] 

Functional Description

 Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. 

Sequence Annotation

 MOD_RES 493 493 Phosphotyrosine (By similarity).
 MOD_RES 538 538 Phosphotyrosine (By similarity).
 MOD_RES 563 563 Phosphoserine (By similarity).
 LIPID 38 38 S-palmitoyl cysteine (By similarity).
 CARBOHYD 205 205 N-linked (GlcNAc...) (Potential).
 CARBOHYD 215 215 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Glycoprotein; Lipoprotein; Membrane; Palmitate; Phosphoprotein; Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 573 AA 

Protein Sequence

Gene Ontology

 GO:0043198; C:dendritic shaft; IDA:RGD.
 GO:0043197; C:dendritic spine; IDA:RGD.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IDA:RGD.
 GO:0042734; C:presynaptic membrane; IDA:RGD.
 GO:0005314; F:high-affinity glutamate transmembrane transporter activity; IMP:RGD.
 GO:0017153; F:sodium:dicarboxylate symporter activity; IEA:InterPro.
 GO:0006836; P:neurotransmitter transport; TAS:RGD. 

Interpro

 IPR001991; Na-dicarboxylate_symporter.
 IPR018107; Na-dicarboxylate_symporter_CS. 

Pfam

 PF00375; SDF 

SMART

  

PROSITE

 PS00713; NA_DICARBOXYL_SYMP_1
 PS00714; NA_DICARBOXYL_SYMP_2 

PRINTS

 PR00173; EDTRNSPORT.