CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003405
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Isochorismatase 
Protein Synonyms/Alias
 2,3 dihydro-2,3 dihydroxybenzoate synthase; Enterobactin synthase component B; Enterochelin synthase B; Isochorismate lyase 
Gene Name
 entB 
Gene Synonyms/Alias
 entG; b0595; JW0587 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
21SHDIPQNKVDWAFEPacetylation[1]
123ADDTVLVKWRYSAFHacetylation[1]
139SPLEQMLKESGRNQLacetylation[1]
170DAFMRDIKPFMVADAacetylation[1]
191DEHLMSLKYVAGRSGacetylation[1]
278PTIDAWWKLLSREVKacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Required for production of 2,3-DHB. Also serves as an aryl carrier protein and plays a role in enterobactin assembly. 
Sequence Annotation
 DOMAIN 214 285 Acyl carrier.
 REGION 26 208 Isochorismate lyase.
 MOD_RES 245 245 O-(pantetheine 4'-phosphoryl)serine  
Keyword
 3D-structure; Complete proteome; Enterobactin biosynthesis; Hydrolase; Phosphopantetheine; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 285 AA 
Protein Sequence
MAIPKLQAYA LPESHDIPQN KVDWAFEPQR AALLIHDMQD YFVSFWGENC PMMEQVIANI 60
AALRDYCKQH NIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQKVVD RLTPDADDTV 120
LVKWRYSAFH RSPLEQMLKE SGRNQLIITG VYAHIGCMTT ATDAFMRDIK PFMVADALAD 180
FSRDEHLMSL KYVAGRSGRV VMTEELLPAP IPASKAALRE VILPLLDESD EPFDDDNLID 240
YGLDSVRMMA LAARWRKVHG DIDFVMLAKN PTIDAWWKLL SREVK 285 
Gene Ontology
 GO:0008908; F:isochorismatase activity; IEA:EC.
 GO:0016765; F:transferase activity, transferring alkyl or aryl (other than methyl) groups; IMP:EcoliWiki.
 GO:0009239; P:enterobactin biosynthetic process; IEA:UniProtKB-UniPathway. 
Interpro
 IPR009081; Acyl_carrier_prot-like.
 IPR016291; Isochorismatase.
 IPR000868; Isochorismatase-like. 
Pfam
 PF00857; Isochorismatase
 PF00550; PP-binding 
SMART
  
PROSITE
 PS50075; ACP_DOMAIN
 PS00012; PHOSPHOPANTETHEINE 
PRINTS
 PR01398; ISCHRISMTASE.