CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019421
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MAP/microtubule affinity-regulating kinase 4 
Protein Synonyms/Alias
 MAP/microtubule affinity-regulating kinase-like 1 
Gene Name
 MARK4 
Gene Synonyms/Alias
 KIAA1860; MARKL1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
67RLLRTIGKGNFAKVKubiquitination[1]
92VAIKIIDKTQLNPSSubiquitination[1]
102LNPSSLQKLFREVRIubiquitination[1]
183NIVHRDLKAENLLLDubiquitination[1, 2]
231PELFQGKKYDGPEVDubiquitination[1]
297FLVLNPAKRCTLEQIubiquitination[1]
356KESLTSQKYNEVTATubiquitination[1]
622LFTKLTSKLTRRVADubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
  
Sequence Annotation
 DOMAIN 59 310 Protein kinase.
 DOMAIN 324 368 UBA.
 DOMAIN 703 752 KA1.
 NP_BIND 65 73 ATP (By similarity).
 ACT_SITE 181 181 Proton acceptor (By similarity).
 BINDING 88 88 ATP (By similarity).
 MOD_RES 214 214 Phosphothreonine; by LKB1.  
Keyword
 Alternative splicing; ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 752 AA 
Protein Sequence
MSSRTVLAPG NDRNSDTHGT LGSGRSSDKG PSWSSRSLGA RCRNSIASCP EEQPHVGNYR 60
LLRTIGKGNF AKVKLARHIL TGREVAIKII DKTQLNPSSL QKLFREVRIM KGLNHPNIVK 120
LFEVIETEKT LYLVMEYASA GEVFDYLVSH GRMKEKEARA KFRQIVSAVH YCHQKNIVHR 180
DLKAENLLLD AEANIKIADF GFSNEFTLGS KLDTFCGSPP YAAPELFQGK KYDGPEVDIW 240
SLGVILYTLV SGSLPFDGHN LKELRERVLR GKYRVPFYMS TDCESILRRF LVLNPAKRCT 300
LEQIMKDKWI NIGYEGEELK PYTEPEEDFG DTKRIEVMVG MGYTREEIKE SLTSQKYNEV 360
TATYLLLGRK TEEGGDRGAP GLALARVRAP SDTTNGTSSS KGTSHSKGQR SSSSTYHRQR 420
RHSDFCGPSP APLHPKRSPT STGEAELKEE RLPGRKASCS TAGSGSRGLP PSSPMVSSAH 480
NPNKAEIPER RKDSTSTPNN LPPSMMTRRN TYVCTERPGA ERPSLLPNGK ENSSGTPRVP 540
PASPSSHSLA PPSGERSRLA RGSTIRSTFH GGQVRDRRAG GGGGGGVQNG PPASPTLAHE 600
AAPLPAGRPR PTTNLFTKLT SKLTRRVADE PERIGGPEVT SCHLPWDQTE TAPRLLRFPW 660
SVKLTSSRPP EALMAALRQA TAAARCRCRQ PQPFLLACLH GGAGGPEPLS HFEVEVCQLP 720
RPGLRGVLFR RVAGTALAFR TLVTRISNDL EL 752 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0043005; C:neuron projection; IDA:UniProtKB.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0043015; F:gamma-tubulin binding; IDA:UniProtKB.
 GO:0008017; F:microtubule binding; IDA:UniProtKB.
 GO:0004674; F:protein serine/threonine kinase activity; IDA:UniProtKB.
 GO:0050321; F:tau-protein kinase activity; IDA:UniProtKB.
 GO:0043130; F:ubiquitin binding; NAS:UniProtKB.
 GO:0001578; P:microtubule bundle formation; IEP:UniProtKB.
 GO:0007399; P:nervous system development; IDA:UniProtKB.
 GO:0043068; P:positive regulation of programmed cell death; NAS:UniProtKB. 
Interpro
 IPR001772; KA1_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS.
 IPR000449; UBA/transl_elong_EF1B_N.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 
Pfam
 PF02149; KA1
 PF00069; Pkinase
 PF00627; UBA 
SMART
 SM00220; S_TKc
 SM00165; UBA 
PROSITE
 PS50032; KA1
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST
 PS50030; UBA 
PRINTS