UniProt Accession

 DHC24_HUMAN; Q15392; D3DQ51; Q9HBA8 

Genbank Protein ID

 AF261758; AF398342; AF398336; AF398337; AF398338; AF398339; AF398340; AF398341; D13643; CH471059; CH471059; BC004375; BC011669 

Genbank Nucleotide ID

 AAG17288.1; AAL15644.1; AAL15644.1; AAL15644.1; AAL15644.1; AAL15644.1; AAL15644.1; AAL15644.1; BAA02806.3; EAX06663.1; EAX06664.1; AAH04375.1; AAH11669.1 

Protein Name

 Delta(24)-sterol reductase 

Protein Synonyms/Alias

 24-dehydrocholesterol reductase; 3-beta-hydroxysterol delta-24-reductase; Diminuto/dwarf1 homolog; Seladin-1 

Gene Name

 DHCR24 

Gene Synonyms/Alias

 KIAA0018 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
76	QRVRDIQKQVREWKE	ubiquitination	[1, 2]
87	EWKEQGSKTFMCTGR	ubiquitination	[2]
254	GLEAICAKFTHESQR	ubiquitination	[2, 3, 4, 5, 6]
301	NSIGNYYKPWFFKHV	ubiquitination	[2, 3, 6, 7]
313	KHVENYLKTNREGLE	ubiquitination	[1, 2, 4, 6, 7, 8]
367	PPKISLLKLTQGETL	ubiquitination	[1, 2, 3, 4, 5, 6, 7]
376	TQGETLRKLYEQHHV	ubiquitination	[2, 3, 6]
459	SCMRQLEKFVRSVHG	ubiquitination	[2]
492	FDGSLYHKLREKLGC	ubiquitination	[1, 3, 6]
496	LYHKLREKLGCQDAF	ubiquitination	[2, 5]
509	AFPEVYDKICKAARH	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048] 

Functional Description

 Catalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis. 

Sequence Annotation

 DOMAIN 58 234 FAD-binding PCMH-type.
 NP_BIND 163 175 FAD (Potential).  

Keyword

 Cholesterol biosynthesis; Cholesterol metabolism; Complete proteome; Disease mutation; Endoplasmic reticulum; FAD; Golgi apparatus; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Reference proteome; Signal; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 516 AA 

Protein Sequence

Gene Ontology

 GO:0005856; C:cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005789; C:endoplasmic reticulum membrane; NAS:UniProtKB.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0050614; F:delta24-sterol reductase activity; IEA:EC.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0016628; F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; IDA:MGI.
 GO:0042605; F:peptide antigen binding; IPI:UniProtKB.
 GO:0008762; F:UDP-N-acetylmuramate dehydrogenase activity; IEA:InterPro.
 GO:0042987; P:amyloid precursor protein catabolic process; IEA:Compara.
 GO:0007050; P:cell cycle arrest; NAS:UniProtKB.
 GO:0016044; P:cellular membrane organization; IEA:Compara.
 GO:0006695; P:cholesterol biosynthetic process; ISS:UniProtKB.
 GO:0030539; P:male genitalia development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:UniProtKB.
 GO:0031639; P:plasminogen activation; IEA:Compara.
 GO:0008104; P:protein localization; IEA:Compara.
 GO:0007265; P:Ras protein signal transduction; IEA:Compara.
 GO:1901214; P:regulation of neuron death; NAS:UniProtKB.
 GO:0009725; P:response to hormone stimulus; IEA:Compara.
 GO:0006979; P:response to oxidative stress; IEP:UniProtKB.
 GO:0043588; P:skin development; ISS:UniProtKB. 

Interpro

 IPR016169; CO_DH_flavot_FAD-bd_sub2.
 IPR016166; FAD-bd_2.
 IPR006094; Oxid_FAD_bind_N. 

Pfam

 PF01565; FAD_binding_4 

SMART

  

PROSITE

 PS51387; FAD_PCMH 

PRINTS