CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006204
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribosomal large subunit pseudouridine synthase F 
Protein Synonyms/Alias
 23S rRNA pseudouridine(2604) synthase; rRNA pseudouridylate synthase F; rRNA-uridine isomerase F 
Gene Name
 rluF 
Gene Synonyms/Alias
 yjbC; b4022; JW3982 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
11DSSVRLNKYISESGIacetylation[1]
53VKPGDVVKVNGQLIEacetylation[1]
108FPIGRLDKDSQGLIFacetylation[1]
125NHGDLVNKILRAGNDacetylation[1]
135RAGNDHEKEYLVTVDacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA. Can, to a small extent, also react with uracil-2605. 
Sequence Annotation
 DOMAIN 7 72 S4 RNA-binding.
 REGION 105 108 Interaction with RNA.
 REGION 187 190 Interaction with RNA.
 ACT_SITE 107 107 Nucleophile (Probable).  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Isomerase; Reference proteome; RNA-binding; rRNA processing. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 290 AA 
Protein Sequence
MLPDSSVRLN KYISESGICS RREADRYIEQ GNVFLNGKRA TIGDQVKPGD VVKVNGQLIE 60
PREAEDLVLI ALNKPVGIVS TTEDGERDNI VDFVNHSKRV FPIGRLDKDS QGLIFLTNHG 120
DLVNKILRAG NDHEKEYLVT VDKPITEEFI RGMSAGVPIL GTVTKKCKVK KEAPFVFRIT 180
LVQGLNRQIR RMCEHFGYEV KKLERTRIMN VSLSGIPLGE WRDLTDDELI DLFKLIENSS 240
SEVKPKAKAK PKTAGIKRPV VKMEKTAEKG GRPASNGKRF TSPGRKKKGR 290 
Gene Ontology
 GO:0009982; F:pseudouridine synthase activity; IMP:EcoCyc.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IMP:EcoCyc. 
Interpro
 IPR020103; PsdUridine_synth_cat_dom.
 IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
 IPR018496; PsdUridine_synth_RsuA/RluB_CS.
 IPR006145; PsdUridine_synth_RsuA/RluD.
 IPR002942; S4_RNA-bd. 
Pfam
 PF00849; PseudoU_synth_2
 PF01479; S4 
SMART
 SM00363; S4 
PROSITE
 PS01149; PSI_RSU
 PS50889; S4 
PRINTS