CPLM-009646

Genbank Nucleotide ID

Transformer-2 protein homolog beta

Protein Synonyms/Alias

TRA-2 beta; TRA2-beta; hTRA2-beta; Splicing factor, arginine/serine-rich 10; Transformer-2 protein homolog B

Homo sapiens (Human)

Position	Peptide	Type	References
140	DLREVFSKYGPIADV	ubiquitination	[1, 2, 3, 4, 5, 6]
173	FENVDDAKEAKERAN	ubiquitination	[2]
197	RVDFSITKRPHTPTP	ubiquitination	[1]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. Can either activate or suppress exon inclusion. Acts additively with RBMX to promote exon 7 inclusion of the survival motor neuron SMN2. Activates the splicing of MAPT/Tau exon 10. Alters pre-mRNA splicing patterns by antagonizing the effects of splicing regulators, like RBMX. Binds to the AG-rich SE2 domain in the SMN exon 7 RNA. Binds to pre- mRNA.

DOMAIN 118 196 RRM.
REGION 193 230 Linker.
MOD_RES 2 2 N-acetylserine.
MOD_RES 2 2 Phosphoserine.
MOD_RES 4 4 Phosphoserine.
MOD_RES 14 14 Phosphoserine.
MOD_RES 50 50 Phosphoserine (By similarity).
MOD_RES 52 52 Phosphoserine (By similarity).
MOD_RES 54 54 Phosphoserine (By similarity).
MOD_RES 56 56 Phosphoserine (By similarity).
MOD_RES 83 83 Phosphoserine.
MOD_RES 85 85 Phosphoserine (By similarity).
MOD_RES 95 95 Phosphoserine.
MOD_RES 97 97 Phosphoserine.
MOD_RES 99 99 Phosphoserine.
MOD_RES 103 103 Phosphothreonine.
MOD_RES 201 201 Phosphothreonine.
MOD_RES 203 203 Phosphothreonine.
MOD_RES 241 241 Dimethylated arginine.
MOD_RES 264 264 Phosphoserine (By similarity).
MOD_RES 266 266 Phosphoserine (By similarity).
MOD_RES 284 284 Phosphoserine (By similarity).

3D-structure; Acetylation; Activator; Alternative splicing; Complete proteome; Direct protein sequencing; Methylation; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repressor; RNA-binding.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0003729; F:mRNA binding; IDA:UniProtKB.
GO:0000166; F:nucleotide binding; IEA:InterPro.
GO:0000398; P:mRNA splicing, via spliceosome; IDA:UniProtKB.
GO:0048026; P:positive regulation of mRNA splicing, via spliceosome; IDA:UniProtKB.
GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IDA:UniProtKB.

IPR012677; Nucleotide-bd_a/b_plait.
IPR000504; RRM_dom.