UniProt Accession

 RUVC_ECOLI; P0A814; P24239 

Genbank Protein ID

 X59551; D10165; U00096; AP009048 

Genbank Nucleotide ID

 CAA42128.1; BAA01032.1; AAC74933.1; BAA15674.1 

Protein Name

 Crossover junction endodeoxyribonuclease RuvC 

Protein Synonyms/Alias

 Holliday junction nuclease RuvC; Holliday junction resolvase RuvC 

Gene Name

 ruvC 

Gene Synonyms/Alias

 b1863; JW1852 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
80	KNADSALKLGQARGV	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group. 

Sequence Annotation

 METAL 8 8 Magnesium.
 METAL 67 67 Magnesium.
 METAL 139 139 Magnesium.
 METAL 142 142 Magnesium.  

Keyword

 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 173 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0048476; C:Holliday junction resolvase complex; IDA:EcoCyc.
 GO:0008821; F:crossover junction endodeoxyribonuclease activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0000725; P:recombinational repair; IMP:EcoCyc.
 GO:0071932; P:replication fork reversal; IGI:EcoCyc. 

Interpro

 IPR012337; RNaseH-like_dom.
 IPR020563; X-over_junc_endoDNase_Mg_BS.
 IPR002176; X-over_junc_endoDNase_RuvC. 

Pfam

 PF02075; RuvC 

SMART

  

PROSITE

 PS01321; RUVC 

PRINTS

 PR00696; RSOLVASERUVC.