UniProt Accession

 FBX25_HUMAN; Q8TCJ0; Q6PJ83; Q7Z4V4; Q9UKB8 

Genbank Protein ID

 AB070270; AF419858; BC020249; BC050393; AF174605 

Genbank Nucleotide ID

 BAB85128.1; AAP97293.1; AAH20249.1; AAH50393.1; AAF04526.1 

Protein Name

 F-box only protein 25 

Protein Synonyms/Alias

  

Gene Name

 FBXO25 

Gene Synonyms/Alias

 FBX25 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
17	SPGWSWIKTEDGWKR	ubiquitination	[1]
31	RCESCSQKLERENNR	ubiquitination	[1]
65	EEHEYASKKRKKDHF	ubiquitination	[1]
66	EHEYASKKRKKDHFR	ubiquitination	[1]
85	TQSFYREKWIYVHKE	ubiquitination	[1]
146	SLSGVAQKNYFNILD	ubiquitination	[1, 2, 3]
271	EDRQLWKKLCQYHFA	ubiquitination	[1]
280	CQYHFAEKQFCRHLI	ubiquitination	[1]
310	LQKHYPAKEQYGDTL	ubiquitination	[1]
366	QHFIDLFKF******	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) (By similarity). 

Sequence Annotation

 DOMAIN 226 274 F-box.
 REGION 1 83 Interaction with beta-actin.  

Keyword

 Actin-binding; Alternative splicing; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; Nucleus; Polymorphism; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 367 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019005; C:SCF ubiquitin ligase complex; ISS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; NAS:UniProtKB. 

Interpro

 IPR001810; F-box_dom_cyclin-like. 

Pfam

  

SMART

  

PROSITE

 PS50181; FBOX 

PRINTS